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SMURF1 (NM_181349) Human Over-expression Lysate

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SKU
LC403618
Lyophilized SMURF1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC219536
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_181349, NP_851994
Predicted MW 83.3 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_851994
Locus ID 57154
Cytogenetics 7q22.1
Protein Families WB
Protein Pathways Endocytosis, TGF-beta signaling pathway, Ubiquitin mediated proteolysis
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Other "SMURF1" proteins (5)
SKU Description Size Price
PH319536 SMURF1 MS Standard C13 and N15-labeled recombinant protein (NP_851994) 10 ug
$3,360.00
LC412480 Lyophilized SMURF1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY403618 Transient overexpression lysate of SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2 (as WB positive control) 100 ug
$665.00
LY412480 Transient overexpression lysate of SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 1 (as WB positive control) 100 ug
$665.00
TP319536 Recombinant protein of human SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2, 20 µg 20 ug
$867.00

file-alt Citations

SMURF1 and SMURF2 in Progenitor Cells from Articular Cartilage and Meniscus during Late-Stage Osteoarthritis
Schminke, Kauffmann, Schubert et al
Cartilage (2020)
Cited by 9
AI Summary: In this study, OriGene’s SMURF1 siRNA (SR311389BL) was used to specifically knock down SMURF1 expression in progenitor cells, enabling the researchers to investigate its role in regulating key transcription factors involved in osteoarthritis.
Snippet: ObjectiveThe aim of this study was to investigate the roles of SMURF1 and SMURF2 in progenitor cells from the human knee in late-stage osteoarthritis (OA).DesignWe applied immunohistochemistry, imm...;For transfection of CPCs and MPCs, we used Human-MSC Nucleofactor Kit (VPE-1001;Lonza). siRNA was obtained commercially for SMURF1 (SR311389BL;OriGene) and SMURF2 (SI00134295;Qiagen). We harvested 5 × 105 CPCs and MPCs at passage 4 and resuspended the cells in 100 µL of human chondrocyte nucleofector solution (VPF1001;Lonza) with 10 µL siRNA of SMURF1 or SMURF2 at 0.2 nmol
Bardoxolone ameliorates TGF-β1-associated renal fibrosis through Nrf2/Smad7 elevation
Song, Lee, Ryoo et al
Free Radic. Biol. Med. (2019) 138, 33-42 DOI: 10.1016/j.freeradbiomed.2019.04.033
AI Summary: OriGene’s pCMV6-Smurf1 plasmid was used to overexpress Smurf1 in cells, enabling the authors to investigate the role of Smurf1 in regulating Smad7 levels and its impact on TGF-β1-associated renal fibrosis.
Snippet: Corporation (Daejeon, Korea). For Smurf1 overexpression, pCMV6-Smurf1 (Origene, Rockville, MD, USA) was transfected in cells using Lipofectamine 2000. 2.5. Total RNA extraction and real-time PCR. Total RNAs were isolated
Showing 1-2 of 2 papers.
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