WNT10A (NM_025216) Human Over-expression Lysate
SKU
LY403062
Transient overexpression lysate of wingless-type MMTV integration site family, member 10A (WNT10A) (as WB positive control)
$436.00
4 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC208561 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_025216, NP_079492 |
| Synonyms | OODD; SSPS; STHAG4 |
| Predicted MW | 42.4 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_079492 |
| Locus ID | 80326 |
| Cytogenetics | 2q35 |
| Protein Families | WB |
| Protein Pathways | Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "WNT10A" proteins (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LC403062 | Lyophilized WNT10A HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
Citations
Roles of heparan sulfate sulfation in dentinogenesis
Hayano, Kurosaka, Yanagita et al
J. Biol. Chem. (2012) 287 (15), 12217-29 DOI: 10.1074/jbc.M111.332924
Hayano, Kurosaka, Yanagita et al
J. Biol. Chem. (2012) 287 (15), 12217-29 DOI: 10.1074/jbc.M111.332924
Cited by 8
AI Summary: OriGene’s Wnt10a-containing cell lysate was used in this study to investigate how Wnt10a protein interacts with cell surface heparan sulfate proteoglycans in odontoblasts, elucidating its role in Wnt signaling and dentin matrix production.
Snippet: Sulf1/Sulf2 double null mutant mice exhibit a thin dentin matrix and short roots combined with reduced expression of dentin sialophosphoprotein (Dspp ... after equilibration with buffer A (20 mm Tris, pH 7.4), 35 μl of Wnt10a-containing cell lysate (Origene Technologies, Rockville
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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