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WNT10A (NM_025216) Human Over-expression Lysate

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SKU
LC403062
Lyophilized WNT10A HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC208561
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_025216, NP_079492
Synonyms OODD; SSPS; STHAG4
Predicted MW 42.4 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_079492
Locus ID 80326
Cytogenetics 2q35
Protein Families WB
Protein Pathways Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway
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Other "WNT10A" proteins (1)
SKU Description Size Price
LY403062 Transient overexpression lysate of wingless-type MMTV integration site family, member 10A (WNT10A) (as WB positive control) 100 ug
$436.00

file-alt Citations

Roles of heparan sulfate sulfation in dentinogenesis
Hayano, Kurosaka, Yanagita et al
J. Biol. Chem. (2012) 287 (15), 12217-29 DOI: 10.1074/jbc.M111.332924
Cited by 8
AI Summary: OriGene’s Wnt10a-containing cell lysate was used in this study to investigate how Wnt10a protein interacts with cell surface heparan sulfate proteoglycans in odontoblasts, elucidating its role in Wnt signaling and dentin matrix production.
Snippet: Sulf1/Sulf2 double null mutant mice exhibit a thin dentin matrix and short roots combined with reduced expression of dentin sialophosphoprotein (Dspp ... after equilibration with buffer A (20 mm Tris, pH 7.4), 35 μl of Wnt10a-containing cell lysate (Origene Technologies, Rockville
Showing 1-1 of 1 papers.
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