MBNL1 (NM_021038) Human Over-expression Lysate
SKU
LY402822
Transient overexpression lysate of muscleblind-like (Drosophila) (MBNL1), transcript variant 1 (as WB positive control)
$436.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC208112 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_021038, NP_066368 |
| Synonyms | EXP; MBNL |
| Predicted MW | 41 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_066368 |
| Locus ID | 4154 |
| Cytogenetics | 3q25.1-q25.2 |
| Protein Families | WB |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "MBNL1" proteins (23)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH308112 | MBNL1 MS Standard C13 and N15-labeled recombinant protein (NP_066368) | 10 ug |
$3,360.00
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| PH319704 | MBNL1 MS Standard C13 and N15-labeled recombinant protein (NP_997178) | 10 ug |
$3,360.00
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|
| PH323104 | MBNL1 MS Standard C13 and N15-labeled recombinant protein (NP_997176) | 10 ug |
$3,360.00
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|
| PH323199 | MBNL1 MS Standard C13 and N15-labeled recombinant protein (NP_997180) | 10 ug |
$3,360.00
|
|
| PH323216 | MBNL1 MS Standard C13 and N15-labeled recombinant protein (NP_997179) | 10 ug |
$3,360.00
|
|
| LC402822 | Lyophilized MBNL1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC404075 | Lyophilized MBNL1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC404076 | Lyophilized MBNL1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC404077 | Lyophilized MBNL1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC404078 | Lyophilized MBNL1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC404079 | Lyophilized MBNL1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC404080 | Lyophilized MBNL1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY404075 | Transient overexpression lysate of muscleblind-like (Drosophila) (MBNL1), transcript variant 2 (as WB positive control) | 100 ug |
$436.00
|
|
| LY404076 | Transient overexpression lysate of muscleblind-like (Drosophila) (MBNL1), transcript variant 3 (as WB positive control) | 100 ug |
$436.00
|
|
| LY404077 | Transient overexpression lysate of muscleblind-like (Drosophila) (MBNL1), transcript variant 4 (as WB positive control) | 100 ug |
$436.00
|
|
| LY404078 | Transient overexpression lysate of muscleblind-like (Drosophila) (MBNL1), transcript variant 5 (as WB positive control) | 100 ug |
$436.00
|
|
| LY404079 | Transient overexpression lysate of muscleblind-like (Drosophila) (MBNL1), transcript variant 6 (as WB positive control) | 100 ug |
$436.00
|
|
| LY404080 | Transient overexpression lysate of muscleblind-like (Drosophila) (MBNL1), transcript variant 7 (as WB positive control) | 100 ug |
$436.00
|
|
| TP308112 | Recombinant protein of human muscleblind-like (Drosophila) (MBNL1), transcript variant 1, 20 µg | 20 ug |
$737.00
|
|
| TP319704 | Recombinant protein of human muscleblind-like (Drosophila) (MBNL1), transcript variant 5, 20 µg | 20 ug |
$737.00
|
|
| TP323104 | Purified recombinant protein of Homo sapiens muscleblind-like (Drosophila) (MBNL1), transcript variant 3, 20 µg | 20 ug |
$737.00
|
|
| TP323199 | Purified recombinant protein of Homo sapiens muscleblind-like (Drosophila) (MBNL1), transcript variant 7, 20 µg | 20 ug |
$737.00
|
|
| TP323216 | Recombinant protein of human muscleblind-like (Drosophila) (MBNL1), transcript variant 6, 20 µg | 20 ug |
$737.00
|
|
Citations
(CCUG)n RNA toxicity in a Drosophila model for myotonic dystrophy type 2 (DM2) activates apoptosis
Yenigun, Sirito, Amcheslavky et al
Dis Model Mech (2017)
Yenigun, Sirito, Amcheslavky et al
Dis Model Mech (2017)
AI Summary: OriGene products, specifically the human MBNL1 and CUGBP1/CELF1 TrueClone expression clones, were used to study the effects of these proteins on RNA toxicity and mis-splicing in a Drosophila model of myotonic dystrophy type 2.
Snippet: Generation of MBNL1 expression clone The human MBNL1 clone was obtained from OriGene (TrueClone accession number ... … Generation of CUGBP1/CELF1 expression clone The human CUGBP1/CELF1 clone was obtained from OriGene (TrueClone accession ...;... Generation of MBNL1 expression clone The human MBNL1 clone was obtained from OriGene (TrueClone accession number ... … Generation of CUGBP1/CELF1 expression clone The human CUGBP1/CELF1 clone was obtained from OriGene (TrueClone accession
Showing 1-1 of 1 papers.
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