HIPPI (IFT57) (NM_018010) Human Over-expression Lysate
SKU
LY402637
Transient overexpression lysate of intraflagellar transport 57 homolog (Chlamydomonas) (IFT57) (as WB positive control)
$436.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC204116 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_018010, NP_060480 |
| Synonyms | ESRRBL1; HIPPI; MHS4R2; OFD18 |
| Predicted MW | 49.1 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_060480 |
| Locus ID | 55081 |
| Cytogenetics | 3q13.12-q13.13 |
| Protein Families | WB |
| Protein Pathways | Huntington's disease |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "HIPPI" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH304116 | IFT57 MS Standard C13 and N15-labeled recombinant protein (NP_060480) | 10 ug |
$3,360.00
|
|
| LC402637 | Lyophilized IFT57 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP304116 | Recombinant protein of human intraflagellar transport 57 homolog (Chlamydomonas) (IFT57), 20 µg | 20 ug |
$867.00
|
|
Citations
BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment
Hsu, Garrison, Kim et al
PLoS Genet. (2017) 13 (10), e1007057 DOI: 10.1371/journal.pgen.1007057
Hsu, Garrison, Kim et al
PLoS Genet. (2017) 13 (10), e1007057 DOI: 10.1371/journal.pgen.1007057
AI Summary: OriGene’s IFT57 overexpression lysate (catalog #LC402637) was used as a positive control in this study to validate protein expression analyses related to BBSome function in photoreceptor outer segment morphogenesis and maintenance.
Snippet: …Cached;IFT57 (Proteintech, #11083, 1:1000 dilution), MACF1 (Proteintech, #13058, 1:1000 dilution), actin (rabbit, Sigma-Aldrich, #A5060, 1:2000 dilution;mouse, Abcam, #6276, 1:3000 dilution). IFT57 over expression lysate used as a positive control was purchased from Origene, Rockville, MD (#LC402637)
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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