SLC39A4 (NM_017767) Human Over-expression Lysate
SKU
LY402614
Transient overexpression lysate of solute carrier family 39 (zinc transporter), member 4 (SLC39A4), transcript variant 1 (as WB positive control)
$665.00
2 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC215229 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_017767, NP_060237 |
| Synonyms | AEZ; AWMS2; ZIP4 |
| Predicted MW | 66 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_060237 |
| Locus ID | 55630 |
| Cytogenetics | 8q24.3 |
| Protein Families | WB |
Reviews
Documents
| Product Manuals |
| FAQs |
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Resources
| Recombinant Protein Resources |
Other "SLC39A4" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH309463 | SLC39A4 MS Standard C13 and N15-labeled recombinant protein (NP_570901) | 10 ug |
$3,360.00
|
|
| LC402614 | Lyophilized SLC39A4 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP309463 | Recombinant protein of human solute carrier family 39 (zinc transporter), member 4 (SLC39A4), transcript variant 2, 20 µg | 20 ug |
$737.00
|
|
Citations
Zip4 mediated zinc influx stimulates insulin secretion in pancreatic Beta cells
Hardy, Prentice, Froese et al
PLoS ONE (2015) 10 (3), e0119136 DOI: 10.1371/journal.pone.0119136
Hardy, Prentice, Froese et al
PLoS ONE (2015) 10 (3), e0119136 DOI: 10.1371/journal.pone.0119136
Cited by 36
AI Summary: OriGene's pCMV6-Zip4GFP (Slc39a4 NM_028064 Mouse cDNA ORF Clone) plasmid was used to over-express Zip4 in MIN6 beta cells, enabling the study to demonstrate that Zip4 mediates zinc influx and stimulates insulin secretion in vitro.
Snippet: Slc39a4 overexpression in MIN6 cells. The Slc39a4 (Zip4) gene was over-expressed using a pCMV6 plasmid carrying Slc39a4 fused to turbo GFP (pCMV6-Zip4GFP;Origene;Slc39a4 NM_028064 Mouse cDNA ORF Clone). ... Cite SaveSaving
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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