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SLC39A4 (NM_017767) Human Over-expression Lysate

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SKU
LC402614
Lyophilized SLC39A4 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC215229
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_017767, NP_060237
Synonyms AEZ; AWMS2; ZIP4
Predicted MW 66 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_060237
Locus ID 55630
Cytogenetics 8q24.3
Protein Families WB
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Other "SLC39A4" proteins (3)
SKU Description Size Price
PH309463 SLC39A4 MS Standard C13 and N15-labeled recombinant protein (NP_570901) 10 ug
$3,360.00
LY402614 Transient overexpression lysate of solute carrier family 39 (zinc transporter), member 4 (SLC39A4), transcript variant 1 (as WB positive control) 100 ug
$665.00
TP309463 Recombinant protein of human solute carrier family 39 (zinc transporter), member 4 (SLC39A4), transcript variant 2, 20 µg 20 ug
$737.00

file-alt Citations

Zip4 mediated zinc influx stimulates insulin secretion in pancreatic Beta cells
Hardy, Prentice, Froese et al
PLoS ONE (2015) 10 (3), e0119136 DOI: 10.1371/journal.pone.0119136
Cited by 36
AI Summary: OriGene's pCMV6-Zip4GFP (Slc39a4 NM_028064 Mouse cDNA ORF Clone) plasmid was used to over-express Zip4 in MIN6 beta cells, enabling the study to demonstrate that Zip4 mediates zinc influx and stimulates insulin secretion in vitro.
Snippet: Slc39a4 overexpression in MIN6 cells. The Slc39a4 (Zip4) gene was over-expressed using a pCMV6 plasmid carrying Slc39a4 fused to turbo GFP (pCMV6-Zip4GFP;Origene;Slc39a4 NM_028064 Mouse cDNA ORF Clone). ... Cite SaveSaving
FigureFigureFigure
Showing 1-1 of 1 papers.
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