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SIRT7 (NM_016538) Human Over-expression Lysate

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SKU
LY402562
Transient overexpression lysate of sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae) (SIRT7) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC205658
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_016538, NP_057622
Synonyms SIR2L7
Predicted MW 44.9 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_057622
Locus ID 51547
Cytogenetics 17q25.3
Protein Families WB
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Other "SIRT7" proteins (3)
SKU Description Size Price
PH305658 SIRT7 MS Standard C13 and N15-labeled recombinant protein (NP_057622) 10 ug
$3,360.00
LC402562 Lyophilized SIRT7 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP305658 Recombinant protein of human sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae) (SIRT7), 20 µg 20 ug
$867.00

file-alt Citations

Sirtuin 7 is Decreased in Pulmonary Fibrosis and Regulates the Fibrotic Phenotype of Lung Fibroblasts
Wyman, Noor, Fishelevich et al
Am. J. Physiol. Lung Cell Mol. Physiol. (2017)
AI Summary: OriGene products—including the GFP-tagged human SIRT7 plasmid and the non-coding vehicle control plasmid—were used in this study to manipulate SIRT7 expression in lung fibroblasts, enabling the authors to investigate SIRT7's role in pulmonary fibrosis.
Snippet: 131 encoding plasmid, GFP-tagged human SIRT7 plasmid (SIRT7-GFP), and non-coding vehicle 132 control plasmid with a similar backbone, all from Origene (Rockville, MD). For SIRT7 silencing, 133 Page 8. 8 transfections ... All 2 versions Cite SaveSaving
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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