SIRT7 (NM_016538) Human Tagged ORF Clone

SKU
RC205658
SIRT7 (Myc-DDK-tagged)-Human sirtuin 7 (SIRT7)
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$686.00
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SIRT7
Synonyms SIR2L7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205658 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCCGGGGGTCTGAGCCGCTCCGAGCGCAAAGCGGCGGAGCGGGTCCGGAGGTTGCGGGAGGAGC
AGCAGAGGGAGCGCCTCCGCCAGGTGTCGCGCATCCTGAGGAAGGCGGCGGCGGAGCGCAGCGCCGAGGA
GGGCCGGCTGCTGGCCGAGAGCGCGGACCTGGTAACGGAGCTGCAGGGCCGGAGCCGGCGGCGCGAGGGC
CTGAAGCGGCGGCAGGAGGAGGTGTGCGACGACCCGGAGGAGCTGCGGGGGAAGGTCCGGGAGCTGGCCA
GCGCCGTCCGGAACGCCAAATACTTGGTCGTCTACACAGGCGCGGGAATCAGCACGGCAGCGTCTATCCC
AGACTACCGGGGCCCTAATGGAGTGTGGACACTGCTTCAGAAAGGGAGAAGCGTTAGTGCTGCCGACCTG
AGCGAGGCCGAGCCAACCCTCACCCACATGAGCATCACCCGTCTGCATGAGCAGAAGCTGGTGCAGCATG
TGGTGTCTCAGAACTGTGACGGGCTCCACCTGAGGAGTGGGCTGCCGCGCACGGCCATCTCCGAGCTCCA
CGGGAACATGTACATTGAAGTCTGTACCTCCTGCGTTCCCAACAGGGAGTACGTGCGGGTGTTCGATGTG
ACGGAGCGCACTGCCCTCCACAGACACCAGACAGGCCGGACCTGCCACAAGTGTGGGACCCAGCTGCGGG
ACACCATTGTGCACTTTGGGGAGAGGGGGACGTTGGGGCAGCCTCTGAACTGGGAAGCGGCGACCGAGGC
TGCCAGCAGAGCAGACACCATCCTGTGTCTAGGGTCCAGCCTGAAGGTTCTAAAGAAGTACCCACGCCTC
TGGTGCATGACCAAGCCCCCTAGCCGGCGGCCGAAGCTTTACATCGTGAACCTGCAGTGGACCCCGAAGG
ATGACTGGGCTGCCCTGAAGCTACATGGGAAGTGTGATGACGTCATGCGGCTCCTCATGGCCGAGCTGGG
CTTGGAGATCCCCGCCTATAGCAGGTGGCAGGATCCCATTTTCTCACTGGCGACTCCCCTGCGTGCTGGT
GAAGAAGGCAGCCACAGTCGGAAGTCGCTGTGCAGAAGCAGAGAGGAGGCCCCGCCTGGGGACCGGGGTG
CACCGCTTAGCTCGGCCCCCATCCTAGGGGGCTGGTTTGGCAGGGGCTGCACAAAACGCACAAAAAGGAA
GAAAGTGACG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC205658 protein sequence
Red=Cloning site Green=Tags(s)

MAAGGLSRSERKAAERVRRLREEQQRERLRQVSRILRKAAAERSAEEGRLLAESADLVTELQGRSRRREG
LKRRQEEVCDDPEELRGKVRELASAVRNAKYLVVYTGAGISTAASIPDYRGPNGVWTLLQKGRSVSAADL
SEAEPTLTHMSITRLHEQKLVQHVVSQNCDGLHLRSGLPRTAISELHGNMYIEVCTSCVPNREYVRVFDV
TERTALHRHQTGRTCHKCGTQLRDTIVHFGERGTLGQPLNWEAATEAASRADTILCLGSSLKVLKKYPRL
WCMTKPPSRRPKLYIVNLQWTPKDDWAALKLHGKCDDVMRLLMAELGLEIPAYSRWQDPIFSLATPLRAG
EEGSHSRKSLCRSREEAPPGDRGAPLSSAPILGGWFGRGCTKRTKRKKVT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_016538
ORF Size 1200 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016538.1, NP_057622.1
RefSeq Size 1749 bp
RefSeq ORF 1203 bp
Locus ID 51547
UniProt ID Q9NRC8
Cytogenetics 17q25.3
Domains SIR2
Protein Families Druggable Genome, Transcription Factors
MW 44.9 kDa
Summary This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008]
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SKU Description Size Price
RC205658L1 Lenti ORF clone of Human sirtuin 7 (SIRT7), Myc-DDK-tagged 10 ug
$986.00
RC205658L2 Lenti ORF clone of Human sirtuin 7 (SIRT7), mGFP tagged 10 ug
$986.00
RC205658L3 Lenti ORF clone of Human sirtuin 7 (SIRT7), Myc-DDK-tagged 10 ug
$986.00
RC205658L4 Lenti ORF clone of Human sirtuin 7 (SIRT7), mGFP tagged 10 ug
$986.00
RG205658 SIRT7 (tGFP-tagged) - Human sirtuin 7 (SIRT7) 10 ug
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SC319613 SIRT7 (untagged)-Human sirtuin 7 (SIRT7) 10 ug
$686.00

Citations

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