SIRT7 (NM_016538) Human Tagged ORF Clone
SKU
RC205658
SIRT7 (Myc-DDK-tagged)-Human sirtuin 7 (SIRT7)
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TrueORF Gold
Protein expression verified by Western blot, fully sequenced and in stock
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Product Data | |
Type | Human Tagged ORF Clone |
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Target Symbol | SIRT7 |
Synonyms | SIR2L7 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
ORF Nucleotide Sequence
>RC205658 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCCGGGGGTCTGAGCCGCTCCGAGCGCAAAGCGGCGGAGCGGGTCCGGAGGTTGCGGGAGGAGC AGCAGAGGGAGCGCCTCCGCCAGGTGTCGCGCATCCTGAGGAAGGCGGCGGCGGAGCGCAGCGCCGAGGA GGGCCGGCTGCTGGCCGAGAGCGCGGACCTGGTAACGGAGCTGCAGGGCCGGAGCCGGCGGCGCGAGGGC CTGAAGCGGCGGCAGGAGGAGGTGTGCGACGACCCGGAGGAGCTGCGGGGGAAGGTCCGGGAGCTGGCCA GCGCCGTCCGGAACGCCAAATACTTGGTCGTCTACACAGGCGCGGGAATCAGCACGGCAGCGTCTATCCC AGACTACCGGGGCCCTAATGGAGTGTGGACACTGCTTCAGAAAGGGAGAAGCGTTAGTGCTGCCGACCTG AGCGAGGCCGAGCCAACCCTCACCCACATGAGCATCACCCGTCTGCATGAGCAGAAGCTGGTGCAGCATG TGGTGTCTCAGAACTGTGACGGGCTCCACCTGAGGAGTGGGCTGCCGCGCACGGCCATCTCCGAGCTCCA CGGGAACATGTACATTGAAGTCTGTACCTCCTGCGTTCCCAACAGGGAGTACGTGCGGGTGTTCGATGTG ACGGAGCGCACTGCCCTCCACAGACACCAGACAGGCCGGACCTGCCACAAGTGTGGGACCCAGCTGCGGG ACACCATTGTGCACTTTGGGGAGAGGGGGACGTTGGGGCAGCCTCTGAACTGGGAAGCGGCGACCGAGGC TGCCAGCAGAGCAGACACCATCCTGTGTCTAGGGTCCAGCCTGAAGGTTCTAAAGAAGTACCCACGCCTC TGGTGCATGACCAAGCCCCCTAGCCGGCGGCCGAAGCTTTACATCGTGAACCTGCAGTGGACCCCGAAGG ATGACTGGGCTGCCCTGAAGCTACATGGGAAGTGTGATGACGTCATGCGGCTCCTCATGGCCGAGCTGGG CTTGGAGATCCCCGCCTATAGCAGGTGGCAGGATCCCATTTTCTCACTGGCGACTCCCCTGCGTGCTGGT GAAGAAGGCAGCCACAGTCGGAAGTCGCTGTGCAGAAGCAGAGAGGAGGCCCCGCCTGGGGACCGGGGTG CACCGCTTAGCTCGGCCCCCATCCTAGGGGGCTGGTTTGGCAGGGGCTGCACAAAACGCACAAAAAGGAA GAAAGTGACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205658 protein sequence
Red=Cloning site Green=Tags(s) MAAGGLSRSERKAAERVRRLREEQQRERLRQVSRILRKAAAERSAEEGRLLAESADLVTELQGRSRRREG LKRRQEEVCDDPEELRGKVRELASAVRNAKYLVVYTGAGISTAASIPDYRGPNGVWTLLQKGRSVSAADL SEAEPTLTHMSITRLHEQKLVQHVVSQNCDGLHLRSGLPRTAISELHGNMYIEVCTSCVPNREYVRVFDV TERTALHRHQTGRTCHKCGTQLRDTIVHFGERGTLGQPLNWEAATEAASRADTILCLGSSLKVLKKYPRL WCMTKPPSRRPKLYIVNLQWTPKDDWAALKLHGKCDDVMRLLMAELGLEIPAYSRWQDPIFSLATPLRAG EEGSHSRKSLCRSREEAPPGDRGAPLSSAPILGGWFGRGCTKRTKRKKVT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
Chromatograms
![]() Sequencher program is needed, download here |
Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_016538 |
ORF Size | 1200 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_016538.1, NP_057622.1 |
RefSeq Size | 1749 bp |
RefSeq ORF | 1203 bp |
Locus ID | 51547 |
UniProt ID | Q9NRC8 |
Cytogenetics | 17q25.3 |
Domains | SIR2 |
Protein Families | Druggable Genome, Transcription Factors |
MW | 44.9 kDa |
Summary | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008] |
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Product Manuals |
FAQs |
SDS |
SKU | Description | Size | Price | |
---|---|---|---|---|
RC205658L1 | Lenti ORF clone of Human sirtuin 7 (SIRT7), Myc-DDK-tagged | 10 ug |
$986.00
|
|
RC205658L2 | Lenti ORF clone of Human sirtuin 7 (SIRT7), mGFP tagged | 10 ug |
$986.00
|
|
RC205658L3 | Lenti ORF clone of Human sirtuin 7 (SIRT7), Myc-DDK-tagged | 10 ug |
$986.00
|
|
RC205658L4 | Lenti ORF clone of Human sirtuin 7 (SIRT7), mGFP tagged | 10 ug |
$986.00
|
|
RG205658 | SIRT7 (tGFP-tagged) - Human sirtuin 7 (SIRT7) | 10 ug |
$886.00
|
|
SC319613 | SIRT7 (untagged)-Human sirtuin 7 (SIRT7) | 10 ug |
$686.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.