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ZNF521 (NM_015461) Human Over-expression Lysate

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SKU
LY402451
Transient overexpression lysate of zinc finger protein 521 (ZNF521) (as WB positive control)
  $665.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC217463
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_015461, NP_056276
Synonyms EHZF; Evi3
Predicted MW 147.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_056276
Locus ID 25925
Cytogenetics 18q11.2
Protein Families WB
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Other "ZNF521" proteins (2)
SKU Description Size Price
LC402451 Lyophilized ZNF521 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP761996 Purified recombinant protein of Human zinc finger protein 521 (ZNF521),Lys1077-Glu1150, with N-terminal His-ABP tag, expressed in E. coli, 50ug 50 ug
$270.00

file-alt Citations

The stem cell-associated transcription co-factor, ZNF521, interacts with GLI1 and GLI2 and enhances the activity of the Sonic hedgehog pathway
Scicchitano, Giordano, Lucchino et al
Cell Death Dis (2019) 10 (10), 715 DOI: 10.1038/s41419-019-1946-x
AI Summary: OriGene contributed to this study by providing the TrueORF Gold human ZNF521 (pCMV6-ZNF521-Myc-DDK) expression plasmid, which was used to investigate the role of ZNF521 in enhancing Sonic hedgehog pathway activity.
Snippet: ,39 as well as TrueORF-Gold human {"type":"entrez-nucleotide","attrs":{"text":"NM_015461.2","term_id":"219801668","term_text":"NM_015461.2"}}NM_015461.2 [/nuccore/NM_015461.2] pCMV6-_ZNF521_-Myc-DDK (Origene) and the mouse cDNA clones pCMV-3xHA-_Zfp521_37, Image Clone pCMV-Sport6-_Zfp521_ (_Evi3_) {"type":"entrez-nucleotide","attrs":{"text":"NM_145492.4","term_id":"293651570","term_text":"NM_14549;;... ,39 as well as TrueORF-Gold human {"type":"entrez-nucleotide","attrs":{"text":"NM_015461.2","term_id":"219801668","term_text":"NM_015461.2"}}NM_015461.2 [/nuccore/NM_015461.2] pCMV6-_ZNF521_-Myc-DDK (Origene) and the mouse cDNA clones pCMV-3xHA-_Zfp521_37, Image Clone pCMV-Sport6-_Zfp521_ (_Evi3_) {"type":"entrez-nucleotide","attrs":{"text":"NM_145492.4","term_id":"293651570","term_text":"NM_14549
FigureFigureFigure
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