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ZNF521 (NM_015461) Human Over-expression Lysate

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SKU
LC402451
Lyophilized ZNF521 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC217463
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_015461, NP_056276
Synonyms EHZF; Evi3
Predicted MW 147.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_056276
Locus ID 25925
Cytogenetics 18q11.2
Protein Families WB
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Other "ZNF521" proteins (2)
SKU Description Size Price
LY402451 Transient overexpression lysate of zinc finger protein 521 (ZNF521) (as WB positive control) 100 ug
$665.00
TP761996 Purified recombinant protein of Human zinc finger protein 521 (ZNF521),Lys1077-Glu1150, with N-terminal His-ABP tag, expressed in E. coli, 50ug 50 ug
$270.00

file-alt Citations

The stem cell-associated transcription co-factor, ZNF521, interacts with GLI1 and GLI2 and enhances the activity of the Sonic hedgehog pathway
Scicchitano, Giordano, Lucchino et al
Cell Death Dis (2019) 10 (10), 715 DOI: 10.1038/s41419-019-1946-x
AI Summary: OriGene contributed to this study by providing the TrueORF Gold human ZNF521 (pCMV6-ZNF521-Myc-DDK) expression plasmid, which was used to investigate the role of ZNF521 in enhancing Sonic hedgehog pathway activity.
Snippet: ,39 as well as TrueORF-Gold human {"type":"entrez-nucleotide","attrs":{"text":"NM_015461.2","term_id":"219801668","term_text":"NM_015461.2"}}NM_015461.2 [/nuccore/NM_015461.2] pCMV6-_ZNF521_-Myc-DDK (Origene) and the mouse cDNA clones pCMV-3xHA-_Zfp521_37, Image Clone pCMV-Sport6-_Zfp521_ (_Evi3_) {"type":"entrez-nucleotide","attrs":{"text":"NM_145492.4","term_id":"293651570","term_text":"NM_14549;;... ,39 as well as TrueORF-Gold human {"type":"entrez-nucleotide","attrs":{"text":"NM_015461.2","term_id":"219801668","term_text":"NM_015461.2"}}NM_015461.2 [/nuccore/NM_015461.2] pCMV6-_ZNF521_-Myc-DDK (Origene) and the mouse cDNA clones pCMV-3xHA-_Zfp521_37, Image Clone pCMV-Sport6-_Zfp521_ (_Evi3_) {"type":"entrez-nucleotide","attrs":{"text":"NM_145492.4","term_id":"293651570","term_text":"NM_14549
FigureFigureFigure
Showing 1-1 of 1 papers.
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