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ZDHHC17 (NM_015336) Human Over-expression Lysate

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SKU
LY402425
Transient overexpression lysate of zinc finger, DHHC-type containing 17 (ZDHHC17) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC207982
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_015336, NP_056151
Synonyms DHHC-17; HIP3; HIP14; HSPC294; HYPH
Predicted MW 72.6 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_056151
Locus ID 23390
Cytogenetics 12q21.2
Protein Families WB
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Resources
Other "ZDHHC17" proteins (1)
SKU Description Size Price
LC402425 Lyophilized ZDHHC17 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Activation of JNK and p38 MAPK Mediated by ZDHHC17 Drives Glioblastoma Multiforme Development and Malignant Progression
Chen, Hao, Li et al
Theranostics (2020) 10 (3), 998-1015 DOI: 10.7150/thno.40076
Cited by 79
AI Summary: OriGene products MAP2K4 siRNA (#SR304323) and ZDHHC17 siRNA (#SR323571) were used in this study to specifically knock down the expression of MAP2K4 and ZDHHC17, enabling investigation of their roles in MAPK pathway activation and glioblastoma progression.
Snippet: The cells were transfected with Negative, MAP2K4 (#SR304323, Origene) or ZDHHC17 (#SR323571, Origene) Stealth siRNA per manufacturer instruction using Lipofectamine RNAiMAX reagent for knockdown experimentation
FigureFigureFigure
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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