ZDHHC17 (NM_015336) Human Over-expression Lysate
SKU
LC402425
Lyophilized ZDHHC17 HEK293T cell transient overexpression lysate (as WB positive control)
$206.00
2 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC207982 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_015336, NP_056151 |
| Synonyms | DHHC-17; HIP3; HIP14; HSPC294; HYPH |
| Predicted MW | 72.6 kDa |
| Components | 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Shipping | Blue Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution Method | The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel. The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. |
| Reference Data | |
| RefSeq | NP_056151 |
| Locus ID | 23390 |
| Cytogenetics | 12q21.2 |
| Protein Families | WB |
Reviews
Documents
| Product Manuals |
| FAQs |
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Resources
| Recombinant Protein Resources |
Other "ZDHHC17" proteins (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LY402425 | Transient overexpression lysate of zinc finger, DHHC-type containing 17 (ZDHHC17) (as WB positive control) | 100 ug |
$436.00
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Citations
Activation of JNK and p38 MAPK Mediated by ZDHHC17 Drives Glioblastoma Multiforme Development and Malignant Progression
Chen, Hao, Li et al
Theranostics (2020) 10 (3), 998-1015 DOI: 10.7150/thno.40076
Chen, Hao, Li et al
Theranostics (2020) 10 (3), 998-1015 DOI: 10.7150/thno.40076
Cited by 79
AI Summary: OriGene products MAP2K4 siRNA (#SR304323) and ZDHHC17 siRNA (#SR323571) were used in this study to specifically knock down the expression of MAP2K4 and ZDHHC17, enabling investigation of their roles in MAPK pathway activation and glioblastoma progression.
Snippet: The cells were transfected with Negative, MAP2K4 (#SR304323, Origene) or ZDHHC17 (#SR323571, Origene) Stealth siRNA per manufacturer instruction using Lipofectamine RNAiMAX reagent for knockdown experimentation
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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