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NFYC (NM_014223) Human Over-expression Lysate

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SKU
LY402291
Transient overexpression lysate of nuclear transcription factor Y, gamma (NFYC), transcript variant 2 (as WB positive control)
  $436.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200060
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_014223, NP_055038
Synonyms CBF-C; CBFC; H1TF2A; HAP5; HSM; NF-YC
Predicted MW 37.2 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_055038
Locus ID 4802
Cytogenetics 1p34.2
Protein Families WB
Protein Pathways Antigen processing and presentation
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Other "NFYC" proteins (13)
SKU Description Size Price
PH300060 NFYC MS Standard C13 and N15-labeled recombinant protein (NP_055038) 10 ug
$3,360.00
PH326680 NFYC MS Standard C13 and N15-labeled recombinant protein (NP_001136061) 10 ug
$3,360.00
LC402291 Lyophilized NFYC HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC428190 Lyophilized NFYC HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC428191 Lyophilized NFYC HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC428192 Lyophilized NFYC HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC428193 Lyophilized NFYC HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY428190 Transient overexpression lysate of nuclear transcription factor Y, gamma (NFYC), transcript variant 3 (as WB positive control) 100 ug
$436.00
LY428191 Transient overexpression lysate of nuclear transcription factor Y, gamma (NFYC), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY428192 Transient overexpression lysate of nuclear transcription factor Y, gamma (NFYC), transcript variant 4 (as WB positive control) 100 ug
$436.00
LY428193 Transient overexpression lysate of nuclear transcription factor Y, gamma (NFYC), transcript variant 5 (as WB positive control) 100 ug
$436.00
TP300060 Recombinant protein of human nuclear transcription factor Y, gamma (NFYC), transcript variant 2, 20 µg 20 ug
$867.00
TP326680 Purified recombinant protein of Homo sapiens nuclear transcription factor Y, gamma (NFYC), transcript variant 4, 20 µg 20 ug
$867.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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