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SOX10 (NM_006941) Human Over-expression Lysate

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SKU
LY402065
Transient overexpression lysate of SRY (sex determining region Y)-box 10 (SOX10) (as WB positive control)
  $436.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC203545
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_006941, NP_008872
Synonyms DOM; PCWH; WS2E; WS4; WS4C
Predicted MW 49.9 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_008872
Locus ID 6663
Cytogenetics 22q13.1
Protein Families WB
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Other "SOX10" proteins (3)
SKU Description Size Price
PH303545 SOX10 MS Standard C13 and N15-labeled recombinant protein (NP_008872) 10 ug
$3,360.00
LC402065 Lyophilized SOX10 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP303545 Recombinant protein of human SRY (sex determining region Y)-box 10 (SOX10), 20 µg 20 ug
$737.00

file-alt Citations

m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade
Yang, Wei, Cui et al
Nat Commun (2019) 10 (1), 2782 DOI: 10.1038/s41467-019-10669-0
AI Summary: In this study, OriGene products such as the FTO shRNA (TL313064) and FTO cDNA clone (RC208447) were used to manipulate FTO expression in melanoma cells, enabling the authors to investigate how FTO regulates tumor growth and response to anti-PD-1 immunotherapy.
Snippet: …Knockdown of FTO increases m6A methylation in the critical protumorigenic melanoma cell-intrinsic genes including PD-1 (PDCD1), CXCR4, and SOX10, leading to increased RNA decay through the m6A reader YTHDF2. …
FigureFigureFigure
A rare myelin protein zero (MPZ) variant alters enhancer activity in vitro and in vivo
Antonellis, Dennis, Burzynski et al
PLoS One (2010) 5 (12), e14346 DOI: 10.1371/journal.pone.0014346
Cited by 20
AI Summary: OriGene products contributed to this study by providing the human MPZ cDNA clone (OriGene: SC127496) used in functional assays to analyze the impact of non-coding MPZ variants on gene expression.
FigureFigureFigure
Showing 1-2 of 2 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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