OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

SOX10 (NM_006941) Human Over-expression Lysate

Be the first to review this product
SKU
LC402065
Lyophilized SOX10 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

Frequently Bought Together (3)

TA333906 Rabbit Polyclonal Anti-SOX10 Antibody
100 ul
$585.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "SOX10 or NM_006941"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC203545
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_006941, NP_008872
Synonyms DOM; PCWH; WS2E; WS4; WS4C
Predicted MW 49.9 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_008872
Locus ID 6663
Cytogenetics 22q13.1
Protein Families WB
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "SOX10" proteins (3)
SKU Description Size Price
PH303545 SOX10 MS Standard C13 and N15-labeled recombinant protein (NP_008872) 10 ug
$3,360.00
LY402065 Transient overexpression lysate of SRY (sex determining region Y)-box 10 (SOX10) (as WB positive control) 100 ug
$436.00
TP303545 Recombinant protein of human SRY (sex determining region Y)-box 10 (SOX10), 20 µg 20 ug
$737.00

file-alt Citations

m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade
Yang, Wei, Cui et al
Nat Commun (2019) 10 (1), 2782 DOI: 10.1038/s41467-019-10669-0
AI Summary: In this study, OriGene products such as the FTO shRNA (TL313064) and FTO cDNA clone (RC208447) were used to manipulate FTO expression in melanoma cells, enabling the authors to investigate how FTO regulates tumor growth and response to anti-PD-1 immunotherapy.
Snippet: …Knockdown of FTO increases m6A methylation in the critical protumorigenic melanoma cell-intrinsic genes including PD-1 (PDCD1), CXCR4, and SOX10, leading to increased RNA decay through the m6A reader YTHDF2. …
FigureFigureFigure
A rare myelin protein zero (MPZ) variant alters enhancer activity in vitro and in vivo
Antonellis, Dennis, Burzynski et al
PLoS One (2010) 5 (12), e14346 DOI: 10.1371/journal.pone.0014346
Cited by 20
AI Summary: OriGene products contributed to this study by providing the human MPZ cDNA clone (OriGene: SC127496) used in functional assays to analyze the impact of non-coding MPZ variants on gene expression.
FigureFigureFigure
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products