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Podoplanin (PDPN) (NM_006474) Human Over-expression Lysate

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SKU
LY401944
Transient overexpression lysate of podoplanin (PDPN), transcript variant 1 (as WB positive control)
  • LY401944 is the same product as LY429296.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $436.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC224811
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_006474, NP_006465
Synonyms AGGRUS; GP36; Gp38; GP40; HT1A-1; OTS8; PA2.26; T1A; T1A-2; T1A2; TI1A
Predicted MW 24.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_006465
Locus ID 10630
Cytogenetics 1p36.21
Protein Families WB
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Other "PDPN" proteins (5)
SKU Description Size Price
LC401944 Lyophilized PDPN HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC405041 Lyophilized PDPN HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY405041 Transient overexpression lysate of podoplanin (PDPN), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP720436 Purified recombinant protein of Homo sapiens podoplanin (PDPN), transcript variant 3 10 ug
$220.00
TP762020 Purified recombinant protein of Human podoplanin (PDPN), transcript variant 2,Ala23-Ser162, with N-terminal His tag, expressed in E. coli, 50ug 50 ug
$270.00

file-alt Citations

Wild-Type IDH1 and Mutant IDH1 Opposingly Regulate Podoplanin Expression in Glioma
Sun, Xiao, Zhao et al
Transl Oncol (2020) 13 (4), 100758 DOI: 10.1016/j.tranon.2020.100758
Cited by 25
AI Summary: OriGene's pLenti-C-Myc-DDK-IRES-Puro lentiviral vector was used to generate constructs for overexpressing full-length podoplanin (Pdpn), enabling the authors to study how wild-type and mutant IDH1 regulate Pdpn expression and glioma cell migration.
Snippet: Lentivirus Generation and Infection. Full-length Pdpn (NM_006474.4) was subcloned into the AsiSI and XhoI restriction sites of the lentiviral vector pLenti-C-Myc-DDK-IRES-Puro (Origene), and the sequence was confirmed by Sanger sequencing
FigureFigureFigure
Showing 1-1 of 1 papers.
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