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Podoplanin (PDPN) (NM_006474) Human Tagged ORF Clone

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SKU
RC224811
PDPN (Myc-DDK-tagged)-Human podoplanin (PDPN), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "PDPN or NM_006474"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PDPN
Synonyms AGGRUS; GP36; Gp38; GP40; HT1A-1; OTS8; PA2.26; T1A; T1A-2; T1A2; TI1A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224811 representing NM_006474
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGACTCCGCTCGGAAAGTTCTCAACTGCAAAGTTTGCTGTCCGGCTGCCTAGGGTCTGGGAAGCTC
GGGCACCCTCCCTCTCCGGGGCTCCTGCTCCCACCCCTCCGGCCCCCCCACCGTCGCGCTCCTCCAGGCT
GGGCCTGTGGCCGCGGTGCTTTTTAATTTTCCCCCAGCTCAGAATCTTGCTGCTCGGCCCCCAGGAGAGC
AACAACTCAACGGGAACGATGTGGAAGGTGTCAGCTCTGCTCTTCGTTTTGGGAAGCGCGTCGCTCTGGG
TCCTGGCAGAAGGAGCCAGCACAGGCCAGCCAGAAGATGACACTGAGACTACAGGTTTGGAAGGCGGCGT
TGCCATGCCAGGTGCCGAAGATGATGTGGTGACTCCAGGAACCAGCGAAGACCGCTATAAGTCTGGCTTG
ACAACTCTGGTGGCAACAAGTGTCAACAGTGTAACAGGCATTCGCATCGAGGATCTGCCAACTTCAGAAA
GCACAGTCCACGCGCAAGAACAAAGTCCAAGCGCCACAGCCTCAAACGTGGCCACCAGTCACTCCACGGA
GAAAGTGGATGGAGACACACAGACAACAGTTGAGAAAGATGGTTTGTCAACAGTGACCCTGGTTGGAATC
ATAGTTGGGGTCTTACTAGCCATCGGCTTCATTGGTGGAATCATCGTTGTGGTTATGCGAAAAATGTCGG
GAAGGTACTCGCCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC224811 representing NM_006474
Red=Cloning site Green=Tags(s)

MLTPLGKFSTAKFAVRLPRVWEARAPSLSGAPAPTPPAPPPSRSSRLGLWPRCFLIFPQLRILLLGPQES
NNSTGTMWKVSALLFVLGSASLWVLAEGASTGQPEDDTETTGLEGGVAMPGAEDDVVTPGTSEDRYKSGL
TTLVATSVNSVTGIRIEDLPTSESTVHAQEQSPSATASNVATSHSTEKVDGDTQTTVEKDGLSTVTLVGI
IVGVLLAIGFIGGIIVVVMRKMSGRYSP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006474
ORF Size 714 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006474.3
RefSeq Size 2828 bp
RefSeq ORF 489 bp
Locus ID 10630
UniProt ID Q86YL7
Cytogenetics 1p36.21
Protein Families Druggable Genome, Transmembrane
MW 24.7 kDa
Summary This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified. provided by RefSeq, Jul 2008
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Documents
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"NM_006474" in other vectors (6)
SKU Description Size Price
RC224811L1 Lenti ORF clone of Human podoplanin (PDPN), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC224811L2 Lenti ORF clone of Human podoplanin (PDPN), transcript variant 1, mGFP tagged 10 ug
$600.00
RC224811L3 Lenti ORF clone of Human podoplanin (PDPN), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC224811L4 Lenti ORF clone of Human podoplanin (PDPN), transcript variant 1, mGFP tagged 10 ug
$600.00
RG224811 PDPN (tGFP-tagged) - Human podoplanin (PDPN), transcript variant 1 10 ug
$489.00 $500.00
SC125342 PDPN (untagged)-Human podoplanin (PDPN), transcript variant 1 10 ug
$300.00

Plasmid Map for RC224811

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC224811
Circular map for RC224811

file-alt Citations

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