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NDRG1 (NM_006096) Human Over-expression Lysate

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SKU
LY401840
Transient overexpression lysate of N-myc downstream regulated 1 (NDRG1), transcript variant 2 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201374
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_006096, NP_006087
Synonyms CAP43; CMT4D; DRG-1; DRG1; GC4; HMSNL; NDR1; NMSL; PROXY1; RIT42; RTP; TARG1; TDD5
Predicted MW 42.8 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_006087
Locus ID 10397
Cytogenetics 8q24.22
Protein Families WB
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Other "NDRG1" proteins (8)
SKU Description Size Price
PH301374 NDRG1 MS Standard C13 and N15-labeled recombinant protein (NP_006087) 10 ug
$3,360.00
PH325609 NDRG1 MS Standard C13 and N15-labeled recombinant protein (NP_001128714) 10 ug
$3,360.00
LC401840 Lyophilized NDRG1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC427604 Lyophilized NDRG1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY427604 Transient overexpression lysate of N-myc downstream regulated 1 (NDRG1), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP301374 Recombinant protein of human N-myc downstream regulated 1 (NDRG1), transcript variant 2, 20 µg 20 ug
$737.00
TP325609 Recombinant protein of human N-myc downstream regulated 1 (NDRG1), transcript variant 1, 20 µg 20 ug
$737.00
TP720621 Purified recombinant protein of Human N-myc downstream regulated 1 (NDRG1), transcript variant 2 10 ug
$340.00

file-alt Citations

The proneural gene ASCL1 governs the transcriptional subgroup affiliation in glioblastoma stem cells by directly repressing the mesenchymal gene NDRG1
Narayanan, Gagliardi, Gallotti et al
Cell Death Differ. (2018) 26 (9), 1813-1831 DOI: 10.1038/s41418-018-0248-7
AI Summary: OriGene's NDRG1 cDNA was used in this study to generate lentiviral constructs for overexpression experiments, enabling the researchers to investigate the role of NDRG1 in glioblastoma stem cell differentiation and subgroup specification.
Snippet: cDNAs for human ASCL1 (Upstate, Lake Placid, NY, USA) and NDRG1 (Origene, Rockville, MD, USA) were cloned into the pC.sin.cPPT.PGK.GFP.WPRE11 monocistronic transfer lentiviral vector (LV) in place of the GFP sequence
Showing 1-1 of 1 papers.
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