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NDRG1 (NM_006096) Human Over-expression Lysate

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SKU
LC401840
Lyophilized NDRG1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201374
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_006096, NP_006087
Synonyms CAP43; CMT4D; DRG-1; DRG1; GC4; HMSNL; NDR1; NMSL; PROXY1; RIT42; RTP; TARG1; TDD5
Predicted MW 42.8 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_006087
Locus ID 10397
Cytogenetics 8q24.22
Protein Families WB
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Other "NDRG1" proteins (8)
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file-alt Citations

The proneural gene ASCL1 governs the transcriptional subgroup affiliation in glioblastoma stem cells by directly repressing the mesenchymal gene NDRG1
Narayanan, Gagliardi, Gallotti et al
Cell Death Differ. (2018) 26 (9), 1813-1831 DOI: 10.1038/s41418-018-0248-7
AI Summary: OriGene's NDRG1 cDNA was used in this study to generate lentiviral constructs for overexpression experiments, enabling the researchers to investigate the role of NDRG1 in glioblastoma stem cell differentiation and subgroup specification.
Snippet: cDNAs for human ASCL1 (Upstate, Lake Placid, NY, USA) and NDRG1 (Origene, Rockville, MD, USA) were cloned into the pC.sin.cPPT.PGK.GFP.WPRE11 monocistronic transfer lentiviral vector (LV) in place of the GFP sequence
Showing 1-1 of 1 papers.
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