RACK1 (NM_006098) Human Over-expression Lysate
SKU
LY401838
Transient overexpression lysate of guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1 (GNB2L1) (as WB positive control)
$436.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC205092 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_006098, NP_006089 |
| Synonyms | Gnb2-rs1; GNB2L1; H12.3; HLC-7; PIG21 |
| Predicted MW | 35.1 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_006089 |
| Locus ID | 10399 |
| Cytogenetics | 5q35.3 |
| Protein Families | WB |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "RACK1" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH305092 | GNB2L1 MS Standard C13 and N15-labeled recombinant protein (NP_006089) | 10 ug |
$3,360.00
|
|
| LC401838 | Lyophilized GNB2L1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP305092 | Recombinant protein of human guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1 (GNB2L1), 20 µg | 20 ug |
$737.00
|
|
Citations
Trafficking, localization and degradation of the Na+,HCO3- co-transporter NBCn1 in kidney and breast epithelial cells
Olesen, Vogensen, Axholm et al
Sci Rep (2018) 8 (1), 7435 DOI: 10.1038/s41598-018-25059-7
Olesen, Vogensen, Axholm et al
Sci Rep (2018) 8 (1), 7435 DOI: 10.1038/s41598-018-25059-7
AI Summary: OriGene contributed to this study by providing siRNAs targeting RACK1 (catalog #SR307048) and a mock control oligo (#SR30004), which were used to assess the role of RACK1 in NBCn1 membrane localization in epithelial cells.
Snippet: ynamic interaction sensitive to, e.g. confluence and cell passage number. siRNA transfection Two siRNA’s directed against RACK1 (NM_006098) and corresponding mock control oligo were purchased from OriGene (OriGene Technologies, Rockville, MD, #SR307048 and #SR30004). Cells were 50%-60% confluent upon transfection: medium was replaced with fresh growth medium without penicillin/streptomycin, and
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in shipping.
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