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RACK1 (NM_006098) Human Over-expression Lysate

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SKU
LC401838
Lyophilized GNB2L1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC205092
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_006098, NP_006089
Synonyms Gnb2-rs1; GNB2L1; H12.3; HLC-7; PIG21
Predicted MW 35.1 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_006089
Locus ID 10399
Cytogenetics 5q35.3
Protein Families WB
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Other "RACK1" proteins (3)
SKU Description Size Price
PH305092 GNB2L1 MS Standard C13 and N15-labeled recombinant protein (NP_006089) 10 ug
$3,360.00
LY401838 Transient overexpression lysate of guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1 (GNB2L1) (as WB positive control) 100 ug
$436.00
TP305092 Recombinant protein of human guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1 (GNB2L1), 20 µg 20 ug
$737.00

file-alt Citations

Trafficking, localization and degradation of the Na+,HCO3- co-transporter NBCn1 in kidney and breast epithelial cells
Olesen, Vogensen, Axholm et al
Sci Rep (2018) 8 (1), 7435 DOI: 10.1038/s41598-018-25059-7
AI Summary: OriGene contributed to this study by providing siRNAs targeting RACK1 (catalog #SR307048) and a mock control oligo (#SR30004), which were used to assess the role of RACK1 in NBCn1 membrane localization in epithelial cells.
Snippet: ynamic interaction sensitive to, e.g. confluence and cell passage number. siRNA transfection Two siRNA’s directed against RACK1 (NM_006098) and corresponding mock control oligo were purchased from OriGene (OriGene Technologies, Rockville, MD, #SR307048 and #SR30004). Cells were 50%-60% confluent upon transfection: medium was replaced with fresh growth medium without penicillin/streptomycin, and
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Showing 1-1 of 1 papers.
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