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LRRC32 (NM_005512) Human Over-expression Lysate

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SKU
LY401690
Transient overexpression lysate of leucine rich repeat containing 32 (LRRC32), transcript variant 1 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC209831
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005512, NP_005503
Synonyms CPPRDD; D11S833E; GARP
Predicted MW 72 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_005503
Locus ID 2615
Cytogenetics 11q13.5
Protein Families WB
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Other "LRRC32" proteins (5)
SKU Description Size Price
PH309831 LRRC32 MS Standard C13 and N15-labeled recombinant protein (NP_005503) 10 ug
$3,360.00
LC401690 Lyophilized LRRC32 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC427024 Lyophilized LRRC32 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY427024 Transient overexpression lysate of leucine rich repeat containing 32 (LRRC32), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP309831 Recombinant protein of human leucine rich repeat containing 32 (LRRC32), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

Signal peptide cleavage is essential for surface expression of a regulatory T cell surface protein, leucine rich repeat containing 32 (LRRC32)
Chan, Somani, Young et al
BMC Biochem. (2011) 12, 27 DOI: 10.1186/1471-2091-12-27
Cited by 21
AI Summary: OriGene’s LRRC32 cDNA was used in this study to generate constructs for investigating the role of signal peptide cleavage in the surface expression of LRRC32 on regulatory T cells.
Snippet: The reported sequence was aligned with the reported cDNA sequence of LRRC32 obtained from OriGene using the Vector NTI (Invitrogen ... LRRC32ΔSP, suggesting that LRRC32 is either produced at levels that were undetectable or is rapidly broken down following translation
FigureFigureFigure
Showing 1-1 of 1 papers.
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