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PKC epsilon (PRKCE) (NM_005400) Human Over-expression Lysate

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SKU
LY401658
Transient overexpression lysate of protein kinase C, epsilon (PRKCE) (as WB positive control)
  $665.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC217702
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005400, NP_005391
Synonyms nPKC-epsilon; PKCE
Predicted MW 83.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_005391
Locus ID 5581
Cytogenetics 2p21
Protein Families WB
Protein Pathways Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Tight junction, Type II diabetes mellitus, Vascular smooth muscle contraction
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Other "PKC epsilon" proteins (5)
SKU Description Size Price
PH317702 PRKCE MS Standard C13 and N15-labeled recombinant protein (NP_005391) 10 ug
$3,360.00
LC401658 Lyophilized PRKCE HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP317702 Recombinant protein of human protein kinase C, epsilon (PRKCE), 20 µg 20 ug
$737.00
TP710186 Purified recombinant protein of Human protein kinase C, epsilon (PRKCE), full length, with C-terminal DDK tag, expressed in sf9, 20ug 20 ug
$540.00
TP720143 Recombinant protein of human protein kinase C, epsilon (PRKCE) 10 ug
$275.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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