OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

PODXL (NM_005397) Human Over-expression Lysate

Be the first to review this product
SKU
LY401657
Transient overexpression lysate of podocalyxin-like (PODXL), transcript variant 2 (as WB positive control)
  $436.00
5 Days*
Bulk/Customize

Frequently Bought Together (3)

TA807750 PODXL mouse monoclonal antibody, clone OTI2C6 (formerly 2C6) 100 ul
$447.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "PODXL or NM_005397"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC210816
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005397, NP_005388
Synonyms gp135; Gp200; PC; PCLP; PCLP-1; PDX; PODXL1
Predicted MW 55.4 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_005388
Locus ID 5420
Cytogenetics 7q32.3
Protein Families WB
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "PODXL" proteins (6)
SKU Description Size Price
PH310816 PODXL MS Standard C13 and N15-labeled recombinant protein (NP_005388) 10 ug
$3,360.00
LC401657 Lyophilized PODXL HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP310816 Purified recombinant protein of Homo sapiens podocalyxin-like (PODXL), transcript variant 2, 20 µg 20 ug
$737.00
TP700181 Purified recombinant protein of Homo sapiens podocalyxin-like (PODXL), transcript variant 2, residues Ser23-Pro461, expressed in HEK293 cells. 20 ug
$900.00
TP700182 Purified recombinant protein of Homo sapiens podocalyxin-like (PODXL), transcript variant 2, residues Gln32-Pro461, expressed in HEK293 cells. 20 ug
$900.00
TP762498 Purified recombinant protein of Human podocalyxin-like (PODXL), transcript variant 1, 273Ser-459Ser, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$240.00

file-alt Citations

Proteome-wide epitope mapping of antibodies using ultra-dense peptide arrays
Forsström, Bisławska Axnas, Stengele et al
Mol. Cell Proteomics (2014)
AI Summary: OriGene products, specifically the recombinant proteins MKNK2 (LY413712), RNF214 (LY403969), GPR56 (LY428998), PRY2 (LY424151), CDK2AP2 (LY417039), MS4A8B (LY403116), and STARD13 (LY406044), were used in this study to validate antibody binding and specificity observed in peptide array experiments.
Snippet: MKNK2 LY413712, RNF214 LY403969, GPR56 LY428998, PRY2 LY424151, CDK2AP2 LY417039, MS4A8B LY403116, STARD13 LY406044, all from Origene, Rockville, MD) were run on precast 4-20% CriterionTM TGXTM SDS-PAGE gradient gels (Bio-Rad ... Cite SaveSaving
Membranous expression of podocalyxin-like protein is an independent factor of poor prognosis in urothelial bladder cancer
Boman, Larsson, Segersten et al
Br. J. Cancer (2013) 108 (11), 2321-8 DOI: 10.1038/bjc.2013.215
Cited by 75
AI Summary: OriGene's recombinant myc-DDK-PODXL lysate (LY401657) was used as a positive control in immunohistochemical analyses to assess PODXL expression in urothelial bladder cancer tissues in this study.
Snippet: 5 Science for Life Laboratory, Royal Institute of Technology, AlbaNova University Center, 106 91 Stockholm, Sweden;6 School of ... Two microgram of lysate from HEK293 cells expressing recombinant myc-DDK-PODXL (LY401657;Origene Technologies, Rockville, MD, USA) or
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products