OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

SMAD4 (NM_005359) Human Over-expression Lysate

Be the first to review this product
SKU
LY401648
Transient overexpression lysate of SMAD family member 4 (SMAD4) (as WB positive control)
  $436.00
In Stock*
Bulk/Customize

Frequently Bought Together (3)

TA324984 SMAD4 Antibody (C-term)
400 ul
$645.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "SMAD4 or NM_005359"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201828
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005359, NP_005350
Synonyms DPC4; JIP; MADH4; MYHRS
Predicted MW 60.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_005350
Locus ID 4089
Cytogenetics 18q21.2
Protein Families WB
Protein Pathways Adherens junction, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "SMAD4" proteins (5)
SKU Description Size Price
PH301828 SMAD4 MS Standard C13 and N15-labeled recombinant protein (NP_005350) 10 ug
$3,360.00
LC401648 Lyophilized SMAD4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP301828 Recombinant protein of human SMAD family member 4 (SMAD4), 20 µg 20 ug
$737.00
TP762318 Purified recombinant protein of Human SMAD family member 4 (SMAD4), Ala152-Tyr322, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$260.00
TP762677 Purified recombinant protein of Human SMAD family member 4 (SMAD4), 1Met-195Tyr, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$270.00

file-alt Citations

A comprehensive joint analysis of the long and short RNA transcriptomes of human erythrocytes
Doss, Corcoran, Jima et al
BMC Genomics (2015) 16 (1), 952 DOI: 10.1186/s12864-015-2156-2
Cited by 130
AI Summary: OriGene contributed to this study by providing the SMAD4 cDNA overexpression construct, which was used to investigate the regulatory effects of miR-4732-3p on SMAD4 in erythroid cells.
Snippet: For luciferase reporter constructs, a portion of the 3' untranslated region (UTR) of SMAD2 and SMAD4 flanking the predicted miR-4732-3p binding site was amplified and cloned into the XhoI and NotI sites downstream of Renilla luciferase in the psiCheck-2 vector (Promega). …The SMAD4 cDNA overexpression construct was obtained from Origene, and the SMAD2 cDNA (HsCD00001922) overexpression construct [65] was obtained from DNASU
FigureFigureFigure
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products