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BMI1 (NM_005180) Human Over-expression Lysate

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SKU
LY401584
Transient overexpression lysate of BMI1 polycomb ring finger oncogene (BMI1) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202871
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005180, NP_005171
Synonyms flvi-2/bmi-1; FLVI2/BMI1; PCGF4; RNF51
Predicted MW 36.9 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_005171
Locus ID 648
Cytogenetics 10p12.2
Protein Families WB
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Other "BMI1" proteins (2)
SKU Description Size Price
LC401584 Lyophilized BMI1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP760041 Recombinant protein of human BMI1 polycomb ring finger oncogene (BMI1), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
$270.00

file-alt Citations

Regulation of miRNA biogenesis and histone modification by K63-polyubiquitinated DDX17 controls cancer stem-like features
Kao, Cheng, Wang et al
Cancer Res. (2019)
AI Summary: OriGene products, including the pCMV6-Myc-DDK-DDX17 plasmid, OCT4, SOX2, and BMI1 promoter-driven luciferase reporter constructs, and the p300 construct, were used to investigate the molecular mechanisms by which DDX17 regulates cancer stem-like features in this study.
Snippet: The pCM6-Myc-DDK-DDX17, the OCT4, SOX2, and BMI1 promoter-driven luciferase reporter constructs were purchased from OriGene Technologies, Inc. The p300 construct was from purchased from OriGene (MD, USA). pXP-Huwe1 promoter was constructed using
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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