BMI1 (NM_005180) Human Recombinant Protein

SKU TP760041

Recombinant protein of human BMI1 polycomb ring finger oncogene (BMI1), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding human full-length BMI1
• Tag: N-His
• Predicted MW: 36.9 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 100 mM arginine, 10% glycerol
• Bioactivity: In vitro kinase assay substrate (PMID: 28270146); Binding assay (competitor) (PMID: 29402932)
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_005171
• Locus ID: 648
• UniProt ID: P35226
• Cytogenetics: 10p12.2
• RefSeq Size: 3435
• RefSeq ORF: 978
• Synonyms: flvi-2/bmi-1; FLVI2/BMI1; PCGF4; RNF51
• Summary: This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. [provided by RefSeq, Sep 2015]
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors