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SLIT2 (NM_004787) Human Over-expression Lysate

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SKU
LY401507
Transient overexpression lysate of slit homolog 2 (Drosophila) (SLIT2) (as WB positive control)
  $665.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC220894
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004787, NP_004778
Synonyms SLIL3; Slit-2
Predicted MW 169.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_004778
Locus ID 9353
Cytogenetics 4p15.31
Protein Families WB
Protein Pathways Axon guidance
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Other "SLIT2" proteins (3)
SKU Description Size Price
PH320894 SLIT2 MS Standard C13 and N15-labeled recombinant protein (NP_004778) 10 ug
$3,360.00
LC401507 Lyophilized SLIT2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP320894 Recombinant protein of human slit homolog 2 (Drosophila) (SLIT2), 20 µg 20 ug
$737.00

file-alt Citations

The role of Slit2 as a tumor suppressor in thyroid cancer
Jeon, Lim, You et al
Mol. Cell. Endocrinol. (2019) 483, 87-96 DOI: 10.1016/j.mce.2019.01.010
AI Summary: OriGene’s Slit2 open reading frame cDNA clones were used in this study to overexpress Slit2 in thyroid cancer cells, enabling the investigation of Slit2’s tumor suppressor role.
Snippet: 2.1. Reagents. Media and cell culture reagents were purchased from GIBCO (Grand Island, NY). Slit2 open reading frame cDNA clones were purchased from Origene (Rockville, MD, USA) and shRNA lentiviral vectors were purchased from Sigma-Aldrich (Darmstadt
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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