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WNT5A (NM_003392) Human Over-expression Lysate

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SKU
LY401155
Transient overexpression lysate of wingless-type MMTV integration site family, member 5A (WNT5A) (as WB positive control)
  $436.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC209206
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_003392, NP_003383
Synonyms hWNT5A
Predicted MW 42.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_003383
Locus ID 7474
Cytogenetics 3p14.3
Protein Families WB
Protein Pathways Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway
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Other "WNT5A" proteins (3)
SKU Description Size Price
PH309206 WNT5A MS Standard C13 and N15-labeled recombinant protein (NP_003383) 10 ug
$3,360.00
LC401155 Lyophilized WNT5A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP309206 Recombinant protein of human wingless-type MMTV integration site family, member 5A (WNT5A), 20 µg 20 ug
$867.00

file-alt Citations

Role of Wnt5a in suppressing invasiveness of hepatocellular carcinoma via epithelial-mesenchymal transition
Wakizaka, Kamiyama, Wakayama et al
Oncology letters (2020) 20 (5), 268 DOI: 10.3892/ol.2020.12131
Cited by 9
AI Summary: OriGene’s Wnt5a shRNA lentiviral particles (TL320572) were used to knock down Wnt5a expression in HCC cell lines, enabling the study to assess the role of Wnt5a in regulating hepatocellular carcinoma invasiveness and epithelial-mesenchymal transition.
Snippet: Cell transfection To establish Wnt5a knockdown cells, we used a lentiviral short hairpin RNA (shRNA) targeting Wnt5a (TL320572;OriGene). … To create overexpressing cells, we obtained the full-length cDNA encoding human Wnt5a from cDNA of Huh7 cells by reverse transcription polymerase chain reaction. The Wnt5a primers were: Forward, 5′-CAGTGTGGTGGAATTGCCACCATGAAGAAGTCC-3′ and reverse, 5′-GATATCTGCAGAATTCTACTTGCACACAAACTGG-3′. … HLF cells were seeded in cell culture dishes, and after reaching 70% confluence, they were transfected with pcDNA3.1-Wnt5a overexpression vector. …
Interleukin-1β induces differentiation of human mesenchymal stem cells into osteoblasts via the Wnt-5a/receptor tyrosine kinase-like orphan receptor 2 pathway
Sonomoto, Yamaoka, Oshita et al
Arthritis Rheum. (2012) 64 (10), 3355-63 DOI: 10.1002/art.34555
Cited by 170
AI Summary: OriGene’s pCMV-Entry vector and pCMV-Entry vector carrying WNT5A cDNA were used in this study to transfect human mesenchymal stem cells, enabling the researchers to investigate the role of Wnt-5a in IL-1β-induced osteoblast differentiation.
Snippet: Both short-term and long-term exposure to inflammatory stimuli such as cytokines often result in tissue damage. ... Vector pCMV-Entry or vector pCMV-Entry carrying WNT5A complementary DNA (cDNA) (OriGene) was transfected using Lipofectamine LTX with Plus reagent
Showing 1-2 of 2 papers.
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