WNT5A (NM_003392) Human Over-expression Lysate
SKU
LC401155
Lyophilized WNT5A HEK293T cell transient overexpression lysate (as WB positive control)
$206.00
2 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC209206 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_003392, NP_003383 |
| Synonyms | hWNT5A |
| Predicted MW | 42.3 kDa |
| Components | 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Shipping | Blue Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution Method | The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel. The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. |
| Reference Data | |
| RefSeq | NP_003383 |
| Locus ID | 7474 |
| Cytogenetics | 3p14.3 |
| Protein Families | WB |
| Protein Pathways | Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "WNT5A" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH309206 | WNT5A MS Standard C13 and N15-labeled recombinant protein (NP_003383) | 10 ug |
$3,360.00
|
|
| LY401155 | Transient overexpression lysate of wingless-type MMTV integration site family, member 5A (WNT5A) (as WB positive control) | 100 ug |
$436.00
|
|
| TP309206 | Recombinant protein of human wingless-type MMTV integration site family, member 5A (WNT5A), 20 µg | 20 ug |
$867.00
|
|
Citations
Role of Wnt5a in suppressing invasiveness of hepatocellular carcinoma via epithelial-mesenchymal transition
Wakizaka, Kamiyama, Wakayama et al
Oncology letters (2020) 20 (5), 268 DOI: 10.3892/ol.2020.12131
Wakizaka, Kamiyama, Wakayama et al
Oncology letters (2020) 20 (5), 268 DOI: 10.3892/ol.2020.12131
Cited by 9
AI Summary: OriGene’s Wnt5a shRNA lentiviral particles (TL320572) were used to knock down Wnt5a expression in HCC cell lines, enabling the study to assess the role of Wnt5a in regulating hepatocellular carcinoma invasiveness and epithelial-mesenchymal transition.
Snippet: Cell transfection To establish Wnt5a knockdown cells, we used a lentiviral short hairpin RNA (shRNA) targeting Wnt5a (TL320572;OriGene). … To create overexpressing cells, we obtained the full-length cDNA encoding human Wnt5a from cDNA of Huh7 cells by reverse transcription polymerase chain reaction. The Wnt5a primers were: Forward, 5′-CAGTGTGGTGGAATTGCCACCATGAAGAAGTCC-3′ and reverse, 5′-GATATCTGCAGAATTCTACTTGCACACAAACTGG-3′. … HLF cells were seeded in cell culture dishes, and after reaching 70% confluence, they were transfected with pcDNA3.1-Wnt5a overexpression vector. …
Interleukin-1β induces differentiation of human mesenchymal stem cells into osteoblasts via the Wnt-5a/receptor tyrosine kinase-like orphan receptor 2 pathway
Sonomoto, Yamaoka, Oshita et al
Arthritis Rheum. (2012) 64 (10), 3355-63 DOI: 10.1002/art.34555
Sonomoto, Yamaoka, Oshita et al
Arthritis Rheum. (2012) 64 (10), 3355-63 DOI: 10.1002/art.34555
Cited by 170
AI Summary: OriGene’s pCMV-Entry vector and pCMV-Entry vector carrying WNT5A cDNA were used in this study to transfect human mesenchymal stem cells, enabling the researchers to investigate the role of Wnt-5a in IL-1β-induced osteoblast differentiation.
Snippet: Both short-term and long-term exposure to inflammatory stimuli such as cytokines often result in tissue damage. ... Vector pCMV-Entry or vector pCMV-Entry carrying WNT5A complementary DNA (cDNA) (OriGene) was transfected using Lipofectamine LTX with Plus reagent
Showing 1-2 of 2 papers.
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