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SYK (NM_003177) Human Over-expression Lysate

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SKU
LY401101
Transient overexpression lysate of spleen tyrosine kinase (SYK), transcript variant 1 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200413
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_003177, NP_003168
Synonyms IMD82; p72-Syk
Predicted MW 71.9 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_003168
Locus ID 6850
Cytogenetics 9q22.2
Protein Families WB
Protein Pathways B cell receptor signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Natural killer cell mediated cytotoxicity
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Other "SYK" proteins (3)
SKU Description Size Price
PH300413 SYK MS Standard C13 and N15-labeled recombinant protein (NP_003168) 10 ug
$3,360.00
LC401101 Lyophilized SYK HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP300413 Recombinant protein of human spleen tyrosine kinase (SYK), transcript variant 1, 20 µg 20 ug
$737.00

file-alt Citations

Phosphorylated immunoreceptor tyrosine-based activation motifs and integrin cytoplasmic domains activate spleen tyrosine kinase via distinct mechanisms
Antenucci, Hytönen, Ylänne
J. Biol. Chem. (2018)
AI Summary: OriGene Technologies contributed to this study by providing the Spleen tyrosine kinase (Syk) cDNA (UniProt ID: P43405), which was used to express the Syk protein fragment (amino acids 1-635) for biochemical assays investigating Syk activation mechanisms.
Snippet: Spleen tyrosine kinase (EC 2.7.10.2) sequence cDNA (UniProt ID: P43405) was obtained from Origene Technologies Inc, (Rockville, MD) and fragment containing amino acids 1-635 was expressed with cleavable N-terminal 6xHistidine tag (MSGSHHHHHHGSSGENLYFQ
Alzheimer's disease pathological lesions activate the spleen tyrosine kinase
Schweig, Yao, Beaulieu-Abdelahad et al
Acta Neuropathol Commun (2017) 5 (1), 69 DOI: 10.1186/s40478-017-0472-2
AI Summary: OriGene Technologies contributed to this study by providing a human SYK cDNA ORF Clone (NM_003177, transcript variant 1), which was used to generate Syk-overexpressing SH-SY5Y cells for investigating the role of Syk in tau accumulation and hyperphosphorylation.
Snippet: streptomycin/fungizone. Generation of Syk overexpressing SH-SY5Y cells. A human cDNA ORF Clone of the human SYK gene (NM_003177, transcript variant 1) was purchased from OriGene Technologies (MD, USA). The cDNA ... Cached
FigureFigureFigure
Showing 1-2 of 2 papers.
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