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SYK (NM_003177) Human Over-expression Lysate

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SKU
LC401101
Lyophilized SYK HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200413
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_003177, NP_003168
Synonyms IMD82; p72-Syk
Predicted MW 71.9 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_003168
Locus ID 6850
Cytogenetics 9q22.2
Protein Families WB
Protein Pathways B cell receptor signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Natural killer cell mediated cytotoxicity
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Other "SYK" proteins (3)
SKU Description Size Price
PH300413 SYK MS Standard C13 and N15-labeled recombinant protein (NP_003168) 10 ug
$3,360.00
LY401101 Transient overexpression lysate of spleen tyrosine kinase (SYK), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP300413 Recombinant protein of human spleen tyrosine kinase (SYK), transcript variant 1, 20 µg 20 ug
$737.00

file-alt Citations

Phosphorylated immunoreceptor tyrosine-based activation motifs and integrin cytoplasmic domains activate spleen tyrosine kinase via distinct mechanisms
Antenucci, Hytönen, Ylänne
J. Biol. Chem. (2018)
AI Summary: OriGene Technologies contributed to this study by providing the Spleen tyrosine kinase (Syk) cDNA (UniProt ID: P43405), which was used to express the Syk protein fragment (amino acids 1-635) for biochemical assays investigating Syk activation mechanisms.
Snippet: Spleen tyrosine kinase (EC 2.7.10.2) sequence cDNA (UniProt ID: P43405) was obtained from Origene Technologies Inc, (Rockville, MD) and fragment containing amino acids 1-635 was expressed with cleavable N-terminal 6xHistidine tag (MSGSHHHHHHGSSGENLYFQ
Alzheimer's disease pathological lesions activate the spleen tyrosine kinase
Schweig, Yao, Beaulieu-Abdelahad et al
Acta Neuropathol Commun (2017) 5 (1), 69 DOI: 10.1186/s40478-017-0472-2
AI Summary: OriGene Technologies contributed to this study by providing a human SYK cDNA ORF Clone (NM_003177, transcript variant 1), which was used to generate Syk-overexpressing SH-SY5Y cells for investigating the role of Syk in tau accumulation and hyperphosphorylation.
Snippet: streptomycin/fungizone. Generation of Syk overexpressing SH-SY5Y cells. A human cDNA ORF Clone of the human SYK gene (NM_003177, transcript variant 1) was purchased from OriGene Technologies (MD, USA). The cDNA ... Cached
FigureFigureFigure
Showing 1-2 of 2 papers.
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