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SOX2 (NM_003106) Human Over-expression Lysate

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SKU
LY401083
Transient overexpression lysate of SRY (sex determining region Y)-box 2 (SOX2) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200757
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_003106, NP_003097
Synonyms ANOP3; MCOPS3
Predicted MW 34.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_003097
Locus ID 6657
Cytogenetics 3q26.33
Protein Families WB
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Other "SOX2" proteins (3)
SKU Description Size Price
PH300757 SOX2 MS Standard C13 and N15-labeled recombinant protein (NP_003097) 10 ug
$3,360.00
LC401083 Lyophilized SOX2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP300757 Recombinant protein of human SRY (sex determining region Y)-box 2 (SOX2), 20 µg 20 ug
$737.00

file-alt Citations

Functional domain analysis of SOX18 transcription factor using a single-chain variable fragment-based approach
Fontaine, Goodall, Prokop et al
MAbs (2018)
AI Summary: OriGene’s human SOX18 and SOX2 HEK lysates were used as protein sources in immunoblot assays to assess the binding specificity of the engineered F5 monoclonal antibody in this study.
Snippet: The binding of F5 MAb to human SOX18 and human SOX2 expressed in HEK lysates (Origene) was also determined by immunoblot with HEK lysate alone as a control
Oncogenic Sox2 regulates and cooperates with VRK1 in cell cycle progression and differentiation
Moura, Fernández, Marín-Royo et al
Sci Rep (2016) 6, 28532 DOI: 10.1038/srep28532
Cited by 23
AI Summary: OriGene’s pCMV6-myc-Flag-Sox2 plasmid was used in this study to express Sox2 and investigate its regulatory role and cooperation with VRK1 in cell cycle progression and differentiation.
Snippet: Human CCND1 gene promoter was studied with pA3-CyclinD1-Luc (wt −1745 + 134)59. Plasmids expressing macroH2A1.1 macroH2A1.2 and macroH2A2 were from M. Buschbeck43. Sox2: pCMV6-myc-Flag-Sox2 (Origene;Rockville, MD) or pBabe-Sox2. Sox4: pCDNA3.1-Sox4, pCDNA3.1-Sox4(S395X), and pCDNA3.1-Sox4(A350P)60
FigureFigureFigure
Human antigen R binding and regulation of SOX2 mRNA in human mesenchymal stem cells
Carelli, Latorre, Caremoli et al
Mol. Pharmacol. (2015)
Cited by 9
AI Summary: OriGene’s pMirTarget vector containing the SOX2 (NM_003106) 3'-UTR was used for mutagenesis experiments to study how the 3'UTR of SOX2 mRNA interacts with regulatory proteins in human mesenchymal stem cells.
Snippet: was performed using Lipofectamine 2000 (Invitrogen) as recommended by the manufacturer. 5.14 hSOX2 mRNA mutagenesis SOX2 (NM_003106) 3'-UTR cloned in pMirTarget (Origene, Rockville, MD, USA) was mutagenized using the following primers: Page 10. ... Cite SaveSaving
Loss of miR-638 in vitro promotes cell invasion and a mesenchymal-like transition by influencing SOX2 expression in colorectal carcinoma cells
Ma, Pan, Fan et al
Mol. Cancer (2014) 13 (1), 118 DOI: 10.1186/1476-4598-13-118
Cited by 68
AI Summary: OriGene contributed to this study by providing the SOX2 overexpression construct, which was used to investigate the role of SOX2 in colorectal carcinoma cell invasion and mesenchymal-like transition.
Snippet: The SOX2 siRNAs (sense, 5′-GGAAUGGACCUUGUAUAGAUC-3′;anti-sense, 5′-UCUAUACAAGGUCCAUUCCCC-3′) were synthesized by RiboBio (Guangzhou, China), and the SOX2 overexpression construct was obtained from Origene Inc. (Beijing, China ... Cite SaveSaving
Showing 1-4 of 4 papers.
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