SOX2 (NM_003106) Human Tagged ORF Clone
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | SOX2 |
| Synonyms | ANOP3; MCOPS3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_003106 |
| ORF Size | 951 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003106.4 |
| RefSeq Size | 2520 bp |
| RefSeq ORF | 954 bp |
| Locus ID | 6657 |
| UniProt ID | P48431, A0A0U3FYV6 |
| Cytogenetics | 3q26.33 |
| Protein Families | Adult stem cells, Cancer stem cells, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors |
| MW | 34.3 kDa |
| Summary | This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003106" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200757L1 | Lenti ORF clone of Human SRY (sex determining region Y)-box 2 (SOX2), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC200757L2 | Lenti ORF clone of Human SRY (sex determining region Y)-box 2 (SOX2), mGFP tagged | 10 ug |
$750.00
|
|
| RC200757L3 | Lenti ORF clone of Human SRY (sex determining region Y)-box 2 (SOX2), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC200757L4 | Lenti ORF clone of Human SRY (sex determining region Y)-box 2 (SOX2), mGFP tagged | 10 ug |
$750.00
|
|
| RG200757 | SOX2 (tGFP-tagged) - Human SRY (sex determining region Y)-box 2 (SOX2) | 10 ug |
$489.00
$650.00
|
|
| SC303274 | SOX2 (untagged)-Human SRY (sex determining region Y)-box 2 (SOX2) | 10 ug |
$450.00
|
|
Citations
SOX9 and TCF transcription factors associate to mediate Wnt/β-catenin target gene activation in colorectal cancer
Ramakrishnan, Burby, Adiga et al
J Biol Chem (2022) 299 (1), 102735 DOI: 10.1016/j.jbc.2022.102735
Ramakrishnan, Burby, Adiga et al
J Biol Chem (2022) 299 (1), 102735 DOI: 10.1016/j.jbc.2022.102735
Cited by 23
AI Summary: OriGene’s SOX2 ORF clone (RC200757) was used as a template to generate a Flag-SOX2 expression plasmid, facilitating experiments on the role of related transcription factors in Wnt/β-catenin signaling in colorectal cancer cells.
Snippet: sembly. To create a Flag-SOX2 expression plasmid, the Flag-SOX9 expression plasmid was restriction digested with BamHI and XhoI. SOX2 complementary DNA was PCR amplified from a tagged SOX2 ORF clone (Origene, RC200757) using primers containing BamHI and XhoI sites. The PCR product was digested with the two enzymes and ligated into the vector. A sequence encoding the Flag-SRY construct was ordered
Downregulation of SOX2 by inhibition of Usp9X induces apoptosis in melanoma
Potu, Kandarpa, Peterson et al
Oncotarget (2021) 12 (3), 160-172 DOI: 10.18632/oncotarget.27869
Potu, Kandarpa, Peterson et al
Oncotarget (2021) 12 (3), 160-172 DOI: 10.18632/oncotarget.27869
Cited by 18
AI Summary: OriGene’s pCMV6-FLAG-SOX2 plasmid (RC200757) was used in this study to express FLAG-tagged SOX2 in HEK293T cells, enabling investigation of the interaction between SOX2 and Usp9x and the role of SOX2 in melanoma apoptosis.
Snippet: (PARP), anti-pERK (Cell Signaling Technology);anti-HA (clone 3F10;Roche Applied Science). Co-immunoprecipitation for Usp9x and SOX2 Usp9x-HA, FLAG-Usp9x (FL) [20] and FLAG-SOX2 (pCMV6-FLAG-SOX2) (Origene RC200757) plasmids were introduced into HEK293T cells by Lipofectamine (Invitrogen). Cells were collected after 48 hrs and lysates were prepared in buffer containing, 25 mM HEPES (pH 7.5), 400
FOLFOX Therapy Induces Feedback Upregulation of CD44v6 through YB-1 to Maintain Stemness in Colon Initiating Cells
Ghatak, Hascall, Markwald et al
Int J Mol Sci (2021) 22 (2) DOI: 10.3390/ijms22020753
Ghatak, Hascall, Markwald et al
Int J Mol Sci (2021) 22 (2) DOI: 10.3390/ijms22020753
Cited by 27
AI Summary: In this study, OriGene products, specifically the CD44v6 (Cat# SC317380) and YB-1 (Cat# SR303548) CRISPR/Cas9 knockout plasmids, were used to delete CD44v6 and YB-1 genes, enabling the authors to demonstrate their roles in maintaining stemness and drug resistance in colon cancer initiating cells.
Snippet: [36], and aldehyde dehydrogenase (ALDH1) [37] describe CIC characteristics. However, the cell-surface markers that recognize CICs and have a functional role in the antiapoptotic signaling to drive tumorigenesis have remained poorly defined. Our working hypothesis for this study is that CICs are involved in the development of drug-resistance, treatment failure, and tumor relapse in cancer [38,39]
Downregulation of FOXO3a by DNMT1 promotes breast cancer stem cell properties and tumorigenesis
Liu, Song, Qiu et al
Cell Death Differ. (2019) 27 (3), 966-983 DOI: 10.1038/s41418-019-0389-3
Liu, Song, Qiu et al
Cell Death Differ. (2019) 27 (3), 966-983 DOI: 10.1038/s41418-019-0389-3
AI Summary: In this study, OriGene products including the FOXO3a expression plasmid (RC217307) and FOXO3a shRNA (TG313244) were used to manipulate FOXO3a levels, enabling the authors to investigate the role of FOXO3a in breast cancer stem cell properties and tumorigenesis.
Snippet: Downregulation of FOXO3a by DNMT1 promotes breast cancer stem cell properties and tumorigenesis
O-GlcNAc modification of Sox2 regulates self-renewal in pancreatic cancer by promoting its stability
Sharma, Gupta, Dauer et al
Theranostics (2019) 9 (12), 3410-3424 DOI: 10.7150/thno.32615
Sharma, Gupta, Dauer et al
Theranostics (2019) 9 (12), 3410-3424 DOI: 10.7150/thno.32615
AI Summary: OriGene’s Human OGT gene knockout kit (KN206822) was used to generate OGT-knockout pancreatic cancer cell lines, enabling the study of how O-GlcNAc modification of Sox2 regulates self-renewal and tumor recurrence.
Snippet: Generation of OGT-Knockout, Sox2 overexpression and mutant Cell line. Human OGT gene knockout kit was obtained from Origene, Rockville, MD, USA ({"type":"entrez-nucleotide"," attrs":{"text":"KN206822","term_id":"693543064","term_text":"KN206822"}}KN206822
Oncogenic Sox2 regulates and cooperates with VRK1 in cell cycle progression and differentiation
Moura, Fernández, Marín-Royo et al
Sci Rep (2016) 6, 28532 DOI: 10.1038/srep28532
Moura, Fernández, Marín-Royo et al
Sci Rep (2016) 6, 28532 DOI: 10.1038/srep28532
Cited by 23
AI Summary: OriGene’s pCMV6-myc-Flag-Sox2 plasmid was used in this study to express Sox2 and investigate its regulatory role and cooperation with VRK1 in cell cycle progression and differentiation.
Snippet: Human CCND1 gene promoter was studied with pA3-CyclinD1-Luc (wt −1745 + 134)59. Plasmids expressing macroH2A1.1 macroH2A1.2 and macroH2A2 were from M. Buschbeck43. Sox2: pCMV6-myc-Flag-Sox2 (Origene;Rockville, MD) or pBabe-Sox2. Sox4: pCDNA3.1-Sox4, pCDNA3.1-Sox4(S395X), and pCDNA3.1-Sox4(A350P)60
Showing 1-6 of 7 papers.
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