OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Monocarboxylic acid transporter 1 (SLC16A1) (NM_003051) Human Over-expression Lysate

Be the first to review this product
SKU
LY401069
Transient overexpression lysate of solute carrier family 16, member 1 (monocarboxylic acid transporter 1) (SLC16A1), transcript variant 1 (as WB positive control)
  $436.00
In Stock*
Bulk/Customize

Frequently Bought Together (3)

TA321556 Anti-SLC16A1 Rabbit Polyclonal Antibody
100 ul
$305.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "Monocarboxylic acid transporter 1 or NM_003051"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC205213
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_003051, NP_003042
Synonyms HHF7; MCT; MCT1; MCT1D
Predicted MW 53.9 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_003042
Locus ID 6566
Cytogenetics 1p13.2
Protein Families WB
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "Monocarboxylic acid transporter 1" proteins (5)
SKU Description Size Price
PH305213 SLC16A1 MS Standard C13 and N15-labeled recombinant protein (NP_003042) 10 ug
$3,360.00
LC401069 Lyophilized SLC16A1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC433182 Lyophilized SLC16A1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY433182 Transient overexpression lysate of solute carrier family 16, member 1 (monocarboxylic acid transporter 1) (SLC16A1), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP305213 Recombinant protein of human solute carrier family 16, member 1 (monocarboxylic acid transporter 1) (SLC16A1), 20 µg 20 ug
$737.00

file-alt Citations

Identification and Characterization of the Role of REEP5 in Sarco-Endoplasmic Reticulum Formation, Maintenance, and Function in Cardiac Muscle
Lee
Thesis (2021)
Snippet: … Immunodepletion experiments were performed by incubating primary antibodies with 10 μg of purified cMyc/DDK-tagged human FAM162A (Origene, LY415331), cMyc/DDKtagged human MCT1 (Origene, LY401069), or cMyc/DDK-tagged human COX20 (Origene, LY405090) lysates in 10 mL of 5% milk (1μg /mL) for 3 hr at 4°C prior to probing the membrane at 4°C overnight
Bioinformatic analysis of membrane and associated proteins in murine cardiomyocytes and human myocardium
Lee, Hadipour-Lakmehsari, Kim et al
Sci Data (2020) 7 (1), 425 DOI: 10.1038/s41597-020-00762-1
Cited by 20
AI Summary: OriGene products—cMyc/DDK-tagged human FAM162A (LY415331), MCT1 (LY401069), and COX20 (LY405090)—were used as purified protein lysates for immunodepletion experiments to validate antibody specificity in the study.
Snippet: pped immunoblots are available and provided as Supplemental Fig. 9. Immunodepletion experiments were performed by incubating primary antibodies with 10 μg of purified cMyc/DDK-tagged human FAM162A (Origene, LY415331), cMyc/DDK-tagged human MCT1 (Origene, LY401069), or cMyc/DDK-tagged human COX20 (Origene, LY405090) lysates in 10 mL of 5% milk (1μg /mL) for 3 hr at 4°C prior to probing the
FigureFigureFigure
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products