Monocarboxylic acid transporter 1 (SLC16A1) (NM_003051) Human Tagged ORF Clone
SKU
RC205213
SLC16A1 (Myc-DDK-tagged)-Human solute carrier family 16, member 1 (monocarboxylic acid transporter 1) (SLC16A1), transcript variant 1
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Monocarboxylic acid transporter 1 |
| Synonyms | HHF7; MCT; MCT1; MCT1D |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_003051 |
| ORF Size | 1500 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003051.4 |
| RefSeq Size | 3927 bp |
| RefSeq ORF | 1503 bp |
| Locus ID | 6566 |
| UniProt ID | P53985 |
| Cytogenetics | 1p13.2 |
| Protein Families | Transmembrane |
| MW | 53.9 kDa |
| Summary | The protein encoded by this gene is a proton-linked monocarboxylate transporter that catalyzes the movement of many monocarboxylates, such as lactate and pyruvate, across the plasma membrane. Mutations in this gene are associated with erythrocyte lactate transporter defect. Alternatively spliced transcript variants have been found for this gene.provided by RefSeq, Oct 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003051" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC205213L1 | Lenti ORF clone of Human solute carrier family 16, member 1 (monocarboxylic acid transporter 1) (SLC16A1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC205213L2 | Lenti ORF clone of Human solute carrier family 16, member 1 (monocarboxylic acid transporter 1) (SLC16A1), transcript variant 1, mGFP tagged | 10 ug |
$757.00
|
|
| RC205213L3 | Lenti ORF clone of Human solute carrier family 16, member 1 (monocarboxylic acid transporter 1) (SLC16A1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC205213L4 | Lenti ORF clone of Human solute carrier family 16, member 1 (monocarboxylic acid transporter 1) (SLC16A1), transcript variant 1, mGFP tagged | 10 ug |
$757.00
|
|
| RG205213 | SLC16A1 (tGFP-tagged) - Human solute carrier family 16, member 1 (monocarboxylic acid transporter 1) (SLC16A1), transcript variant 1 | 10 ug |
$489.00
$657.00
|
|
| SC118233 | SLC16A1 (untagged)-Human solute carrier family 16, member 1 (monocarboxylic acid transporter 1) (SLC16A1), transcript variant 1 | 10 ug |
$699.00
|
|
Citations
Monocarboxylate transporter MCT1 promotes tumor metastasis independently of its activity as a lactate transporter
Payen, Hsu, Rädecke et al
Cancer Res. (2017)
Payen, Hsu, Rädecke et al
Cancer Res. (2017)
AI Summary: OriGene’s pCMV6/Entry-mct1 plasmid was used to transfect mouse 4T1-shMCT1a cells with human wild-type MCT1 cDNA in rescue experiments, enabling the authors to assess the functional role of MCT1 in cancer cell migration and metastasis.
Snippet: In rescue experiments, mouse 4T1-shMCT1a cells were transfected with human wild-type MCT1 (hMCT1+) cDNA using pCMV6/Entry-mct1 plasmid (OriGene), and selected with G418 (400 µg/mL). For MCT1 deletion, SiHa-CRISPR-MCT1 cells were produced using ...;... In rescue experiments, mouse 4T1-shMCT1a cells were transfected with human wild-type MCT1 (hMCT1+) cDNA using pCMV6/Entry-mct1 plasmid (OriGene), an...
Oligodendrocyte dysfunction in the pathogenesis of amyotrophic lateral sclerosis
Philips, Bento-Abreu, Nonneman et al
Brain (2013) 136 (Pt 2), 471-82 DOI: 10.1093/brain/aws339
Philips, Bento-Abreu, Nonneman et al
Brain (2013) 136 (Pt 2), 471-82 DOI: 10.1093/brain/aws339
Cited by 260
AI Summary: OriGene products contributed to this study by providing FLAG-tagged MCT1 and FLAG-tagged α-tubulin1a constructs, which were used to investigate oligodendrocyte dysfunction in amyotrophic lateral sclerosis.
Snippet: CCL-131) and human embryonic kidney cells (ATCC CRL-1573) were co-transfected using Lipofectamine (Life Technologies) according to the manufacturer instructions. Constructs encoding FLAG-tagged MCT1 and FLAG-tagged α-tubulin1a were purchased from Origene.
Showing 1-2 of 2 papers.
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