Syndecan 4 (SDC4) (NM_002999) Human Over-expression Lysate
$436.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC204619 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_002999, NP_002990 |
| Synonyms | SYND4 |
| Predicted MW | 21.6 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_002990 |
| Locus ID | 6385 |
| Cytogenetics | 20q13.12 |
| Protein Families | WB |
| Protein Pathways | Cell adhesion molecules (CAMs), ECM-receptor interaction |
Reviews
Documents
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Resources
| Recombinant Protein Resources |
Other "Syndecan 4" proteins (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LC401051 | Lyophilized SDC4 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
Citations
SDC4 Gene Silencing Favors Human Papillary Thyroid Carcinoma Cell Apoptosis and Inhibits Epithelial Mesenchymal Transition via Wnt/β-Catenin Pathway
Chen, Gao, Shen et al
Mol. Cells (2018) 41 (9), 853-867 DOI: 10.14348/molcells.2018.0103
Chen, Gao, Shen et al
Mol. Cells (2018) 41 (9), 853-867 DOI: 10.14348/molcells.2018.0103
AI Summary: OriGene's SDC4 over-expression plasmids were used to transfect papillary thyroid carcinoma cells, enabling the study of how SDC4 gene modulation affects cell apoptosis, migration, and the Wnt/β-catenin signaling pathway.
Snippet: The two selected PTC cell lines were transfected and assigned into the blank (cells without any transfection), negative control (NC) (cells transfected with nonsense negative sequences), SDC4 over-expression (cells transfected with SDC4 over-expression plasmids, Beijing OriGene Biotechnology Co., Ltd., China), si-SDC4 (cells transfected with SDC4 interference plasmids), si-β-catenin (cells transfected with β-catenin interference plasmids), SDC4 over-expression + si-NC (cells transfected with SDC4 over-expression + nonsense negative sequences)
Showing 1-1 of 1 papers.
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