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RBP1 (NM_002899) Human Over-expression Lysate

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SKU
LY401017
Transient overexpression lysate of retinol binding protein 1, cellular (RBP1), transcript variant 1 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC214515
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002899, NP_002890
Synonyms CRABP-I; CRBP; CRBP1; CRBPI; RBPC
Predicted MW 15.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_002890
Locus ID 5947
Cytogenetics 3q23
Protein Families WB
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Other "RBP1" proteins (3)
SKU Description Size Price
PH314515 RBP1 MS Standard C13 and N15-labeled recombinant protein (NP_002890) 10 ug
$3,360.00
LC401017 Lyophilized RBP1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP314515 Recombinant protein of human retinol binding protein 1, cellular (RBP1), transcript variant 1, 20 µg 20 ug
$737.00

file-alt Citations

Electronegative low-density lipoprotein of patients with metabolic syndrome induces pathogenesis of aorta through disruption of the stimulated by retinoic acid 6 cascade
Chen, Ke, Chan et al
J Diabetes Investig (2019) 11 (3), 535-544 DOI: 10.1111/jdi.13158
AI Summary: OriGene products, specifically the pCMV6-GFP vector and human crbp1 cDNA, were used for crbp1 gene transfection experiments to assess the role of the STRA6 cascade in atherosclerosis development in this study.
Snippet: occurred. The pCMV6-GFP vector and human crbp1 cDNA (Gene Number NM-002899) was purchased from OriGene Technologies Inc. (Rockville, MD, USA). The crbp1 cDNA was combined with the pCMV6-GFP vector (OriGene Technologies
Showing 1-1 of 1 papers.
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