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Paxillin (PXN) (NM_002859) Human Over-expression Lysate

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SKU
LY401010
Transient overexpression lysate of paxillin (PXN), transcript variant 2 (as WB positive control)
  $665.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC213811
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002859, NP_002850
Predicted MW 60.8 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_002850
Locus ID 5829
Cytogenetics 12q24.23
Protein Families WB
Protein Pathways Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, VEGF signaling pathway
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Other "Paxillin" proteins (8)
SKU Description Size Price
PH313811 PXN MS Standard C13 and N15-labeled recombinant protein (NP_002850) 10 ug
$3,360.00
LC401010 Lyophilized PXN HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC425922 Lyophilized PXN HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC429750 Lyophilized PXN HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY425922 Transient overexpression lysate of paxillin (PXN), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY429750 Transient overexpression lysate of paxillin (PXN), transcript variant 3 (as WB positive control) 100 ug
$436.00
TP313811 Recombinant protein of human paxillin (PXN), transcript variant 2, 20 µg 20 ug
$867.00
TP760834 Purified recombinant protein of Human paxillin (PXN), transcript variant 3, full length, with N-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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