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CKS1 (CKS1B) (NM_001826) Human Over-expression Lysate

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SKU
LY400692
Transient overexpression lysate of CDC28 protein kinase regulatory subunit 1B (CKS1B), transcript variant 1 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202933
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001826, NP_001817
Synonyms CKS1; ckshs1; PNAS-16; PNAS-18
Predicted MW 9.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001817
Locus ID 1163
Cytogenetics 1q21.3
Protein Families WB
Protein Pathways Pathways in cancer, Small cell lung cancer
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Other "CKS1" proteins (3)
SKU Description Size Price
PH302933 CKS1B MS Standard C13 and N15-labeled recombinant protein (NP_001817) 10 ug
$3,360.00
LC400692 Lyophilized CKS1B HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP302933 Recombinant protein of human CDC28 protein kinase regulatory subunit 1B (CKS1B), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

Regulation of microRNA miR-197-3p/CDC28 protein kinase regulatory subunit 1B (CKS1B) axis by Circular RNA hsa_circ_0000285 promotes glioma progression
Deng, Liu, Yang et al
Bioengineered (2022) 13 (3), 4757-4772 DOI: 10.1080/21655979.2022.2031673
Cited by 14
AI Summary: In this study, OriGene products, specifically the anti-Bax antibody (Cat# TA3215) and anti-Bcl-2 antibody (Cat# TA802727), were used in Western blotting analyses to assess apoptosis-related protein expression in glioma cells, thereby contributing to the evaluation of how circ_0000285 regulates glioma progression.
Snippet: , their critical role in gene regulation is being recognized [4]. Through gene regulation, circRNAs are implicated in various biological processes, including cell proliferation, energy metabolism, tumorigenesis, and embryonic development [5]. In glioma, increasing number of circRNAs were described to have pro- or anti-tumoral functions [6,7]. For example, circ-0014359 stimulates the oncogenicity o
Showing 1-1 of 1 papers.
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