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CKS1 (CKS1B) (NM_001826) Human Over-expression Lysate

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SKU
LC400692
Lyophilized CKS1B HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202933
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001826, NP_001817
Synonyms CKS1; ckshs1; PNAS-16; PNAS-18
Predicted MW 9.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_001817
Locus ID 1163
Cytogenetics 1q21.3
Protein Families WB
Protein Pathways Pathways in cancer, Small cell lung cancer
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Other "CKS1" proteins (3)
SKU Description Size Price
PH302933 CKS1B MS Standard C13 and N15-labeled recombinant protein (NP_001817) 10 ug
$3,360.00
LY400692 Transient overexpression lysate of CDC28 protein kinase regulatory subunit 1B (CKS1B), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP302933 Recombinant protein of human CDC28 protein kinase regulatory subunit 1B (CKS1B), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

Regulation of microRNA miR-197-3p/CDC28 protein kinase regulatory subunit 1B (CKS1B) axis by Circular RNA hsa_circ_0000285 promotes glioma progression
Deng, Liu, Yang et al
Bioengineered (2022) 13 (3), 4757-4772 DOI: 10.1080/21655979.2022.2031673
Cited by 14
AI Summary: In this study, OriGene products, specifically the anti-Bax antibody (Cat# TA3215) and anti-Bcl-2 antibody (Cat# TA802727), were used in Western blotting analyses to assess apoptosis-related protein expression in glioma cells, thereby contributing to the evaluation of how circ_0000285 regulates glioma progression.
Snippet: , their critical role in gene regulation is being recognized [4]. Through gene regulation, circRNAs are implicated in various biological processes, including cell proliferation, energy metabolism, tumorigenesis, and embryonic development [5]. In glioma, increasing number of circRNAs were described to have pro- or anti-tumoral functions [6,7]. For example, circ-0014359 stimulates the oncogenicity o
Showing 1-1 of 1 papers.
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