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CD1 (CD1D) (NM_001766) Human Over-expression Lysate

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SKU
LY400670
Transient overexpression lysate of CD1d molecule (CD1D) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC206613
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001766, NP_001757
Synonyms CD1A; R3; R3G1
Predicted MW 37.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001757
Locus ID 912
Cytogenetics 1q23.1
Protein Families WB
Protein Pathways Hematopoietic cell lineage
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Other "CD1" proteins (2)
SKU Description Size Price
LC400670 Lyophilized CD1D HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP700273 Purified recombinant protein of human CD1D molecule (CD1D), transcript variant 1, with C-terminal Fc tag, expressed in human cells, 20 µg 20 ug
$900.00

file-alt Citations

CD1d expression in glioblastoma is a promising target for NKT cell-based cancer immunotherapy
Hara, Koyama-Nasu, Takami et al
Cancer Immunol Immunother (2020)
Cited by 28
AI Summary: OriGene's pCMV6-XLA4/hCD1d plasmid was used to generate CD1d-transfected U87 glioblastoma cells, enabling the study of CD1d's role in NKT cell-mediated cytotoxicity in this research.
Snippet: To generate CD1d-transfected U87 cells, pCMV6-XLA4/hCD1d (OriGene Technologies, Rockville, MD, USA) was transfected into U87 cells using a cationic lipid-based transfection reagents, Lipofectamine 3000 (Thermo Fisher Scientific), according to the respective;- To generate CD1d-transfected U87 cells, pCMV6-XLA4/hCD1d (OriGene Technologies, Rockville, MD, USA) was transfected into U87 cells using a cationic lipid-based transfection reagents, Lipofectamine 3000 (Thermo Fisher Scientific), according to the respective
Activated invariant natural killer T cells directly recognize leukemia cells in a CD1d-independent manner
Aoki, Takami, Takatani et al
Cancer Sci (2020) 111 (7), 2223-2233 DOI: 10.1111/cas.14428
Cited by 23
AI Summary: In this paper, OriGene’s Human CD1d (NM_001766) TrueORF cDNA clone was used to generate CD1d-expressing Jurkat cells, enabling the authors to investigate the CD1d-dependent and -independent recognition of leukemia cells by invariant natural killer T (iNKT) cells.
FigureFigureFigure
Showing 1-2 of 2 papers.
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