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CD1 (CD1D) (NM_001766) Human Over-expression Lysate

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SKU
LC400670
Lyophilized CD1D HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC206613
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001766, NP_001757
Synonyms CD1A; R3; R3G1
Predicted MW 37.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_001757
Locus ID 912
Cytogenetics 1q23.1
Protein Families WB
Protein Pathways Hematopoietic cell lineage
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Other "CD1" proteins (2)
SKU Description Size Price
LY400670 Transient overexpression lysate of CD1d molecule (CD1D) (as WB positive control) 100 ug
$436.00
TP700273 Purified recombinant protein of human CD1D molecule (CD1D), transcript variant 1, with C-terminal Fc tag, expressed in human cells, 20 µg 20 ug
$900.00

file-alt Citations

CD1d expression in glioblastoma is a promising target for NKT cell-based cancer immunotherapy
Hara, Koyama-Nasu, Takami et al
Cancer Immunol Immunother (2020)
Cited by 28
AI Summary: OriGene's pCMV6-XLA4/hCD1d plasmid was used to generate CD1d-transfected U87 glioblastoma cells, enabling the study of CD1d's role in NKT cell-mediated cytotoxicity in this research.
Snippet: To generate CD1d-transfected U87 cells, pCMV6-XLA4/hCD1d (OriGene Technologies, Rockville, MD, USA) was transfected into U87 cells using a cationic lipid-based transfection reagents, Lipofectamine 3000 (Thermo Fisher Scientific), according to the respective;- To generate CD1d-transfected U87 cells, pCMV6-XLA4/hCD1d (OriGene Technologies, Rockville, MD, USA) was transfected into U87 cells using a cationic lipid-based transfection reagents, Lipofectamine 3000 (Thermo Fisher Scientific), according to the respective
Activated invariant natural killer T cells directly recognize leukemia cells in a CD1d-independent manner
Aoki, Takami, Takatani et al
Cancer Sci (2020) 111 (7), 2223-2233 DOI: 10.1111/cas.14428
Cited by 23
AI Summary: In this paper, OriGene’s Human CD1d (NM_001766) TrueORF cDNA clone was used to generate CD1d-expressing Jurkat cells, enabling the authors to investigate the CD1d-dependent and -independent recognition of leukemia cells by invariant natural killer T (iNKT) cells.
FigureFigureFigure
Showing 1-2 of 2 papers.
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