IFIT1 (NM_001548) Human Over-expression Lysate
SKU
LY400592
Transient overexpression lysate of interferon-induced protein with tetratricopeptide repeats 1 (IFIT1), transcript variant 2 (as WB positive control)
$436.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC203320 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_001548, NP_001539 |
| Synonyms | C56; G10P1; IFI-56; IFI-56K; IFI56; IFIT-1; IFNAI1; ISG56; P56; RNM561 |
| Predicted MW | 55.4 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_001539 |
| Locus ID | 3434 |
| Cytogenetics | 10q23.31 |
| Protein Families | WB |
Reviews
Documents
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Resources
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Other "IFIT1" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH303320 | IFIT1 MS Standard C13 and N15-labeled recombinant protein (NP_001539) | 10 ug |
$3,360.00
|
|
| LC400592 | Lyophilized IFIT1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP303320 | Recombinant protein of human interferon-induced protein with tetratricopeptide repeats 1 (IFIT1), transcript variant 2, 20 µg | 20 ug |
$737.00
|
|
Citations
IFIT1 Expression Patterns Induced by H9N2 Virus and Inactivated Viral Particle in Human Umbilical Vein Endothelial Cells and Bronchus Epithelial Cells
Feng, Zhang, Wang et al
Mol. Cells (2018) 41 (4), 271-281 DOI: 10.14348/molcells.2018.2091
Feng, Zhang, Wang et al
Mol. Cells (2018) 41 (4), 271-281 DOI: 10.14348/molcells.2018.2091
AI Summary: OriGene contributed to this study by providing the monoclonal antibody for IFIT1 detection and the peroxidase-conjugated secondary antibody, which were used to analyze IFIT1 protein expression in cell samples.
Snippet: … The membranes were blocked with 5% skim milk and probed with a monoclonal antibody to β-actin (Santa Cruz Biotechnology, USA), IFIT1 (ORIGENE, USA), after a further incubation with peroxidase conjugated secondary antibody (ORIGENE, USA). … The relative protein levels of IFIT1 to β-actin were performed by Image J software
Showing 1-1 of 1 papers.
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