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GDI2 (NM_001494) Human Over-expression Lysate

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SKU
LY400572
Transient overexpression lysate of GDP dissociation inhibitor 2 (GDI2), transcript variant 1 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200596
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001494, NP_001485
Synonyms HEL-S-46e; RABGDIB
Predicted MW 50.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001485
Locus ID 2665
Cytogenetics 10p15.1
Protein Families WB
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Other "GDI2" proteins (5)
SKU Description Size Price
PH300596 GDI2 MS Standard C13 and N15-labeled recombinant protein (NP_001485) 10 ug
$3,360.00
LC400572 Lyophilized GDI2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426526 Lyophilized GDI2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY426526 Transient overexpression lysate of GDP dissociation inhibitor 2 (GDI2), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP300596 Recombinant protein of human GDP dissociation inhibitor 2 (GDI2), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

Adipose tissue depot-specific intracellular and extracellular cues contributing to insulin resistance in obese individuals
Guzmán-Ruiz, Tercero-Alcázar, Rabanal-Ruiz et al
FASEB J. (2020)
AI Summary: OriGene contributed to this study by providing the DsRed-GDI2 plasmid (NM_001494), which was used for overexpression experiments to investigate the role of GDI2 in adipocyte function related to insulin resistance.
Snippet: For overexpression studies, 3T3-L1 adipocytes (D6) on 150-mm Petri dishes were electroporated using either a DsRed or CFP empty vectors or containing the coding sequence of the GDP dissociation inhibitor of Rab proteins, GDI2 (NM_001494;Origene, Madrid, Spain) (DsRed-GDI2) (50 µg), following our established procedures.22,26 Thereafter, cells were cultured for 48 hours, and then, processed for confocal microscopy and functional studies (see below)
Showing 1-1 of 1 papers.
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