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IGFBP1 (NM_000596) Human Over-expression Lysate

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SKU
LY400199
Transient overexpression lysate of insulin-like growth factor binding protein 1 (IGFBP1) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC209268
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000596, NP_000587
Synonyms AFBP; hIGFBP-1; IBP1; IGF-BP25; PP12
Predicted MW 27.9 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000587
Locus ID 3484
Cytogenetics 7p12.3
Protein Families WB
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Other "IGFBP1" proteins (3)
SKU Description Size Price
PH309268 IGFBP1 MS Standard C13 and N15-labeled recombinant protein (NP_000587) 10 ug
$3,360.00
LC400199 Lyophilized IGFBP1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP309268 Recombinant protein of human insulin-like growth factor binding protein 1 (IGFBP1), 20 µg 20 ug
$867.00

file-alt Citations

IGFBP-1 Expression Promotes Tamoxifen Resistance in Breast Cancer Cells via Erk Pathway Activation
Zheng, Sowers, Houston
Front Endocrinol (Lausanne) (2020) 11, 233 DOI: 10.3389/fendo.2020.00233
Cited by 32
AI Summary: OriGene’s Human IGFBP-1 expression vector (NM_000596) and the corresponding empty vector were used to generate breast cancer cell lines with altered IGFBP-1 expression, enabling the study of IGFBP-1’s role in tamoxifen resistance.
Snippet: Human IGFBP-1 expression vector (NM_000596) and the vector devoid of IGFBP-1 ORF were purchased from OriGene (Rockville, MD). Plasmid transfection was performed using Lipofectamine 3000 reagent in serum-free Opti-MEM (Life Technologies, Carlsbad, CA) according to the manufacture's protocol. …
FigureFigureFigure
Inactivation of Stat3 and crosstalk of miRNA155-5p and FOXO3a contribute to the induction of IGFBP1 expression by beta-elemene in human lung cancer
Zheng, Tang, Zheng et al
Exp. Mol. Med. (2018) 50 (9), 121 DOI: 10.1038/s12276-018-0146-6
AI Summary: OriGene products, specifically the PCMV6-AC-GFP control vector and Stat3 overexpression plasmid, were used to manipulate Stat3 expression levels and serve as experimental controls in studying the molecular mechanisms by which β-elemene inhibits lung cancer cell growth.
Snippet: obtained from Life Technologies (Carlsbad, CA, USA). PCMV6-AC-GFP (control vector) and Stat3 overexpression plasmid were obtained from OriGene Technologies (Rockville, MD, USA). β-Elemene, which was purchased from
FigureFigureFigure
Combination of Solamargine and Metformin Strengthens IGFBP1 Gene Expression Through Inactivation of Stat3 and Reciprocal Interaction Between FOXO3a and SP1
Tang, Zheng, Wu et al
Cell. Physiol. Biochem. (2017) 43 (6), 2310-2326 DOI: 10.1159/000484383
AI Summary: In this study, OriGene’s IGFBP1 expression vector (IGFBP1-pCMV6-AC-GFP) and control vector (pCMV-6) were used for transient transfection assays to investigate the role of IGFBP1 in mediating the anti-cancer effects of solamargine and metformin in lung cancer cells.
Snippet: 1), SP1 overexpression pcDNA3.1Sp1/flu vector (kindly provided by Dr. Thomas E. Eling, National Institute of Environmental Health Sciences, USA) [40], the control (pCMV-6) and IGFBP1 expression vectors (IGFBP1-pCMV6-AC-GFP) purchased from OriGene Technologies;... 1), SP1 overexpression pcDNA3.1Sp1/flu vector (kindly provided by Dr. Thomas E. Eling, National Institute of Environmental Health Sciences, USA) [40], the control (pCMV-6) and IGFBP1 expression vectors (IGFBP1-pCMV6-AC-GFP) purchased from OriGene Technologies ...;... 1), SP1 overexpression pcDNA3.1Sp1/flu vector (kindly provided by Dr. Thomas E. Eling, National Institute of Environmental Health Sciences, USA) [40], the control (pCMV-6) and IGFBP1 expression vectors (IGFBP1-pCMV6-AC-GFP) purchased from OriGene Technologies
Complex Behavior of ALDH1A1 and IGFBP1 in Liver Metastasis from a Colorectal Cancer
Kim, Ha, Tak et al
PLoS ONE (2016) 11 (5), e0155160 DOI: 10.1371/journal.pone.0155160
Cited by 36
AI Summary: In this study, OriGene products—specifically, the ALDH1A1 (RC203307) and IGFBP1 (RC203753) expression plasmids—were used to overexpress these genes in colorectal cancer cell lines, enabling the researchers to assess their roles in cell proliferation, invasiveness, and liver metastasis.
Snippet: molecules associated with CLM promote or inhibit tumor progression. For example, the protective nature of autophagy has a dual effect on cancer, acting as a tumor suppressor in the early stages of tumorigenesis but supporting cancer progression in established tumors [
FigureFigureFigure
Showing 1-4 of 4 papers.
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