IGFBP1 (NM_000596) Human Over-expression Lysate
SKU
LC400199
Lyophilized IGFBP1 HEK293T cell transient overexpression lysate (as WB positive control)
$206.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC209268 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_000596, NP_000587 |
| Synonyms | AFBP; hIGFBP-1; IBP1; IGF-BP25; PP12 |
| Predicted MW | 27.9 kDa |
| Components | 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Shipping | Blue Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution Method | The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel. The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. |
| Reference Data | |
| RefSeq | NP_000587 |
| Locus ID | 3484 |
| Cytogenetics | 7p12.3 |
| Protein Families | WB |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "IGFBP1" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH309268 | IGFBP1 MS Standard C13 and N15-labeled recombinant protein (NP_000587) | 10 ug |
$3,360.00
|
|
| LY400199 | Transient overexpression lysate of insulin-like growth factor binding protein 1 (IGFBP1) (as WB positive control) | 100 ug |
$436.00
|
|
| TP309268 | Recombinant protein of human insulin-like growth factor binding protein 1 (IGFBP1), 20 µg | 20 ug |
$867.00
|
|
Citations
IGFBP-1 Expression Promotes Tamoxifen Resistance in Breast Cancer Cells via Erk Pathway Activation
Zheng, Sowers, Houston
Front Endocrinol (Lausanne) (2020) 11, 233 DOI: 10.3389/fendo.2020.00233
Zheng, Sowers, Houston
Front Endocrinol (Lausanne) (2020) 11, 233 DOI: 10.3389/fendo.2020.00233
Cited by 32
AI Summary: OriGene’s Human IGFBP-1 expression vector (NM_000596) and the corresponding empty vector were used to generate breast cancer cell lines with altered IGFBP-1 expression, enabling the study of IGFBP-1’s role in tamoxifen resistance.
Snippet: Human IGFBP-1 expression vector (NM_000596) and the vector devoid of IGFBP-1 ORF were purchased from OriGene (Rockville, MD). Plasmid transfection was performed using Lipofectamine 3000 reagent in serum-free Opti-MEM (Life Technologies, Carlsbad, CA) according to the manufacture's protocol. …
Inactivation of Stat3 and crosstalk of miRNA155-5p and FOXO3a contribute to the induction of IGFBP1 expression by beta-elemene in human lung cancer
Zheng, Tang, Zheng et al
Exp. Mol. Med. (2018) 50 (9), 121 DOI: 10.1038/s12276-018-0146-6
Zheng, Tang, Zheng et al
Exp. Mol. Med. (2018) 50 (9), 121 DOI: 10.1038/s12276-018-0146-6
AI Summary: OriGene products, specifically the PCMV6-AC-GFP control vector and Stat3 overexpression plasmid, were used to manipulate Stat3 expression levels and serve as experimental controls in studying the molecular mechanisms by which β-elemene inhibits lung cancer cell growth.
Snippet: obtained from Life Technologies (Carlsbad, CA, USA). PCMV6-AC-GFP (control vector) and Stat3 overexpression plasmid were obtained from OriGene Technologies (Rockville, MD, USA). β-Elemene, which was purchased from
Combination of Solamargine and Metformin Strengthens IGFBP1 Gene Expression Through Inactivation of Stat3 and Reciprocal Interaction Between FOXO3a and SP1
Tang, Zheng, Wu et al
Cell. Physiol. Biochem. (2017) 43 (6), 2310-2326 DOI: 10.1159/000484383
Tang, Zheng, Wu et al
Cell. Physiol. Biochem. (2017) 43 (6), 2310-2326 DOI: 10.1159/000484383
AI Summary: In this study, OriGene’s IGFBP1 expression vector (IGFBP1-pCMV6-AC-GFP) and control vector (pCMV-6) were used for transient transfection assays to investigate the role of IGFBP1 in mediating the anti-cancer effects of solamargine and metformin in lung cancer cells.
Snippet: 1), SP1 overexpression pcDNA3.1Sp1/flu vector (kindly provided by Dr. Thomas E. Eling, National Institute of Environmental Health Sciences, USA) [40], the control (pCMV-6) and IGFBP1 expression vectors (IGFBP1-pCMV6-AC-GFP) purchased from OriGene Technologies;... 1), SP1 overexpression pcDNA3.1Sp1/flu vector (kindly provided by Dr. Thomas E. Eling, National Institute of Environmental Health Sciences, USA) [40], the control (pCMV-6) and IGFBP1 expression vectors (IGFBP1-pCMV6-AC-GFP) purchased from OriGene Technologies ...;... 1), SP1 overexpression pcDNA3.1Sp1/flu vector (kindly provided by Dr. Thomas E. Eling, National Institute of Environmental Health Sciences, USA) [40], the control (pCMV-6) and IGFBP1 expression vectors (IGFBP1-pCMV6-AC-GFP) purchased from OriGene Technologies
Complex Behavior of ALDH1A1 and IGFBP1 in Liver Metastasis from a Colorectal Cancer
Kim, Ha, Tak et al
PLoS ONE (2016) 11 (5), e0155160 DOI: 10.1371/journal.pone.0155160
Kim, Ha, Tak et al
PLoS ONE (2016) 11 (5), e0155160 DOI: 10.1371/journal.pone.0155160
Cited by 36
AI Summary: In this study, OriGene products—specifically, the ALDH1A1 (RC203307) and IGFBP1 (RC203753) expression plasmids—were used to overexpress these genes in colorectal cancer cell lines, enabling the researchers to assess their roles in cell proliferation, invasiveness, and liver metastasis.
Snippet: molecules associated with CLM promote or inhibit tumor progression. For example, the protective nature of autophagy has a dual effect on cancer, acting as a tumor suppressor in the early stages of tumorigenesis but supporting cancer progression in established tumors [
Showing 1-4 of 4 papers.
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