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Protein C (PROC) (NM_000312) Human Over-expression Lysate

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SKU
LY400117
Transient overexpression lysate of protein C (inactivator of coagulation factors Va and VIIIa) (PROC) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC206582
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000312, NP_000303
Synonyms APC; PC; PROC1; THPH3; THPH4
Predicted MW 52.1 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000303
Locus ID 5624
Cytogenetics 2q14.3
Protein Families WB
Protein Pathways Complement and coagulation cascades
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Other "Protein C" proteins (3)
SKU Description Size Price
PH306582 PROC MS Standard C13 and N15-labeled recombinant protein (NP_000303) 10 ug
$3,360.00
LC400117 Lyophilized PROC HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP306582 Recombinant protein of human protein C (inactivator of coagulation factors Va and VIIIa) (PROC), 20 µg 20 ug
$737.00

file-alt Citations

Tuning the Buffering Capacity of Polyethylenimine with Glycerol Molecules for Efficient Gene Delivery: Staying In or Out of the Endosomes
Singh, Maharjan, Park et al
Macromol Biosci (2015)
Cited by 61
AI Summary: In this study, OriGene’s TurboGFP plasmid was used to monitor protein expression, enabling the researchers to assess the transfection efficiency of their novel polyethylenimine-based gene delivery vectors.
Snippet: USA). HiSpeed plasmid maxi kit for plasmid purification was purchased from Qiagen (USA). TurboGFP (tGFP) encoding green fluorescent protein for monitoring protein expression was bought from Origene (USA). JM109 competent ... Related articles All 3 versions Cite SaveSaving
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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