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Protein C (PROC) (NM_000312) Human Over-expression Lysate

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SKU
LC400117
Lyophilized PROC HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC206582
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000312, NP_000303
Synonyms APC; PC; PROC1; THPH3; THPH4
Predicted MW 52.1 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_000303
Locus ID 5624
Cytogenetics 2q14.3
Protein Families WB
Protein Pathways Complement and coagulation cascades
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Other "Protein C" proteins (3)
SKU Description Size Price
PH306582 PROC MS Standard C13 and N15-labeled recombinant protein (NP_000303) 10 ug
$3,360.00
LY400117 Transient overexpression lysate of protein C (inactivator of coagulation factors Va and VIIIa) (PROC) (as WB positive control) 100 ug
$436.00
TP306582 Recombinant protein of human protein C (inactivator of coagulation factors Va and VIIIa) (PROC), 20 µg 20 ug
$737.00

file-alt Citations

Tuning the Buffering Capacity of Polyethylenimine with Glycerol Molecules for Efficient Gene Delivery: Staying In or Out of the Endosomes
Singh, Maharjan, Park et al
Macromol Biosci (2015)
Cited by 61
AI Summary: In this study, OriGene’s TurboGFP plasmid was used to monitor protein expression, enabling the researchers to assess the transfection efficiency of their novel polyethylenimine-based gene delivery vectors.
Snippet: USA). HiSpeed plasmid maxi kit for plasmid purification was purchased from Qiagen (USA). TurboGFP (tGFP) encoding green fluorescent protein for monitoring protein expression was bought from Origene (USA). JM109 competent ... Related articles All 3 versions Cite SaveSaving
Showing 1-1 of 1 papers.
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